Recombinant Bee Phospholipase A2

• Cat.No.: Pla2-343B
• Product Overview: Recombinant protein produced inE.Colicontains phospholipase P00630 bee venom protein epitopes, 26-162 amino acids. Purified by proprietary chromatographic technique.

Specification

• Cat. No.: Pla2-343B
• Description: Bee venom phospholipase A2 (PLA) is the main allergen in the bee sting allergy. Bee venom phospholipase A2 (BV-PLA2) is a hydrolytic enzyme which specifically cleaves the sn-2 acyl bond of phospholipids at the lipid/water interface. BV-PLA2 is a 14–16-kDa glycoprotein, consisting of 134 amino acids and displaying a single carbohydrate side chain at the residue Asn13. It is also held to be responsible for some systemic anaphylactic reactions in bee venom sensitized individuals. BV-PLA2 presents 3 peptide and a glycopeptide T cell epitopes, which are recognized by both allergic and non-allergic bee venom sensitized subjects. PLA is able to elicit both IgE mediated allergy and normal immunity to bee sting which usually is associated with high affinity IgG4 anti-PLA antibodies.
• Source: E.Coli.
• Form: 10mM Tris-HCl, pH 6.5, 0.1M Sodium Phosphate and 8M urea.
• Purity: Protein is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.
• Applications: Use as an antigen in ELISA and Western Blots.
• Stability: Five years frozen. One month in solution at room temperature.
• Storage: Protein is shipped at ambient temperature. Upon arrival, Store at -20°C.

Gene Information

• Gene Name: Pla2 phospholipase A2 [ Apis mellifera ]
• Synonyms: Pla2; phospholipase A2; GB13351; allergen Api m 1; phosphatidylcholine 2-acylhydrolase; EC 3.1.1.4
• Gene ID: 406141
• mRNA Refseq: NM_001011614
• Protein Refseq: NP_001011614
• UniProt ID: P00630
• Chromosome Location: LG13