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Cat. No. |
BCL2-1346H |
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Product
Overview |
Recombinant
Human Bcl-2 Des BH2 domain (187-202 residues) produced in E.
Coli is a single, non-glycosylated polypeptide chain containing
202 amino acids 1-186 and 203-218. The Bcl-2 is expressed as
His-Tag fusion protein and purified by proprietary
chromatographic techniques. |
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Description |
BCL2 gene
encodes an integral outer mitochondrial membrane protein that
blocks the apoptotic death of some cells such as lymphocytes.
Constitutive expression of BCL2, such as in the case of
translocation of BCL2 to Ig heavy chain locus, is thought to be
the cause of follicular lymphoma. Two transcript variants,
produced by alternate splicing, differ in their C-terminal ends. |
|
Source |
E. Coli. |
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Physical
Appearance |
Sterile
Filtered White lyophilized (freeze-dried) powder. |
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Form |
The protein
contains 10mM Tris-HCL pH-8, 1mM EDTA and 250mM NaCl. |
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Purity |
Greater than
95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by
SDS-PAGE. |
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Applications |
Input marker or
positive control (Western Blotting). Function study. |
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Solubility |
Suspend Bcl-2
in 100μl of 0.5M Acetic acid, over night at 4oC.
Dilute 10 fold into selected buffer system. BCL-2 has tendency
to form intramolecular disulfide bond, 5mM DTT is recommended in
assay buffer. When running SDS-PAGE gel, 10mM DTT is
recommended. |
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Stability |
Lyophilized Bcl-2 although stable
at room temperature for 3 weeks, should be stored desiccated
below -18oC.
Upon reconstitution Bcl-2 should be stored at 4oC
between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein
(0.1% HSA or BSA). |
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Gene
Information
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Gene Name |
BCL2 B-cell CLL/lymphoma 2 [ Homo sapiens ] |
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Synonyms
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Bcl-2;
OTTHUMP00000163680; BCL2; B-cell CLL/lymphoma 2; B-cell lymphoma
protein 2 |
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GeneID |
596 |
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mRNA Refseq |
NM_000633 |
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Protein Refseq |
NP_000624 |
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MIM |
151430 |
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UniProt ID |
P10415 |
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Chromosome
Location |
18q21.33;
18q21.3 |
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Pathway |
Amyotrophic
lateral sclerosis (ALS); Apoptosis; Colorectal cancer; Focal
adhesion; Neurotrophin signaling pathway; Pathways in cancer;
Prostate cancer; Small cell lung cancer |
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Function |
BH3 domain
binding; Protease binding; Protein Heterodimerization activity;
Protein homodimerization activity; Transcription activator
activity; Transcription factor binding |
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PDB rendering based on 1G5M
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Download Datasheet:
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