Recombinant Human CHEK2, GST-tagged, Active

• Cat.No.: CHEK2-282H
• Product Overview: Recombinant human full-length CHK2 were co-expressed by baculovirus inSf9insect cells using an N-terminal GST tag on both proteins. MW=88 kDa.

Specification

• Cat. No.: CHEK2-282H
• Description: CHK2 is rapidly phosphorylated and activated in response to replication blocks and DNA damage; the response to DNA damage occurs in an ataxia telangiectasia mutated (ATM)-dependent manner. Expression of wild-type Chk2 leads to increased p53 stabilization after DNA damage, whereas expression of a dominant-negative Chk2 mutant abrogated both phosphorylation of p53 on Ser-20 and p53 stabilization.
• Source: Sf9 insect cells using baculovirus.
• Sequence: Full-length.
• Applications: Kinase Assay, Western Blot.
• Storage And Stability: Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Information

• Gene Name: CHEK2 CHK2 checkpoint homolog (S. pombe) [ Homo sapiens ]
• Synonyms: CHEK2; CHK2 checkpoint homolog (S. pombe); CDS1; CHK2; LFS2; RAD53; HuCds1; PP1425; CHK2 (checkpoint, S.pombe) homolog; Serine/threonine-protein kinase Chk2;EC2.7.11.1; Cds1
• Gene ID: 11200
• mRNA Refseq: NM_001005735
• Protein Refseq: NP_001005735
• UniProt ID: O96017
• Chromosome Location: 22q12.1
• MIM: 604373
• Pathway: Cell cycle; p53 signaling pathway; Cell Cycle Checkpoints
• Function: ATP binding; nucleotide binding; protein binding; protein serine/threonine kinase activity; transferase activity; magnesium ion binding; protein binding