Recombinant Human Unc-51-Like Kinase 1 (C. elegans), GST-tagged, Active

• Cat.No.: ULK1-255H
• Product Overview: Recombinant human ULK1 (1-649) was expressed bybaculovirusinSf9 insect cellsusing an N-terminal GST tag. MW = 125 kDa.

Specification

• Cat. No.: ULK1-255H
• Description: ULK1 is a serine/threonine protein kinase that plays critical role during initial stages of autophagy which is a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response as well as inhibiting rapamycin-induced autophagy consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.
• Source: Sf9 insect cells using baculovirus.
• Sequence: 1-649.
• Applications: Kinase Assay, Western Blot.
• Storage And Stability: Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Information

• Gene Name: ULK1 unc-51-like kinase 1 (C. elegans) [ Homo sapiens ]
• Synonyms: unc-51-like kinase 1 (C. elegans); ATG1; UNC51; Unc51.1; FLJ38455; FLJ46475; ULK1; Unc-51-like kinase 1; ATG1 autophagy related 1 homolog; EC 2.7.11.1; KIAA0722; unc-51 (C. elegans)-like kinase 1
• Gene ID: 8408
• mRNA Refseq: NM_003565
• Protein Refseq: NP_003556
• MIM: 603168
• UniProt ID: O75385
• Chromosome Location: 12q24.3
• Pathway: Regulation of autophagy; mTOR signaling pathway
• Function: ATP binding; nucleotide binding; protein complex binding; protein serine/threonine kinase activity; transferase activity