Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Antibody > Monoclonal Antibodies > Recombinant Human ACACA, His-tagged

Recombinant Human ACACA, His-tagged

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FLAG/His(1)
His(9)
Cat.No.:
ACACA-26053TH
Product Overview:
Recombinant fragment corresponding to amino acids 39-2346 of Human Acetyl Coenzyme A carboxylase alpha with C terminal His tag expressed in a Baculovirus infected Sf9 cell expression system, 266.5kDa.
Description:
Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5 sequence and encoding distinct isoforms have been found for this gene.
Conjugation:
HIS
Tissue specificity:
Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.
Form:
Liquid
Storage buffer:
Preservative: NoneConstituents: 10% Glycerol, 0.05% Tween 20, 50mM Tris HCl, 3mM DTT, 150mM Sodium chloride, 0.1mM EDTA, 0.1mM EGTA, pH 7.5
Storage:
Store at -20°C, Stable for 6 months at -20°C
Sequence Similarities:
Contains 1 ATP-grasp domain.Contains 1 biotin carboxylation domain.Contains 1 biotinyl-binding domain.Contains 1 carboxyltransferase domain.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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