Recombinant Human 5 -Nucleotidase, Cytosolic II, T7-tagged

• Cat.No.: NT5C2-395H
• Product Overview: Recombinant Human NT5C2 was expressed inE.Coliusing an N-terminal T7 tag. MW=64.5 kDa.

Specification

• Cat. No.: NT5C2-395H
• Description: Purine 5-prime-nucleotidase preferentially hydrolyzes inosine 5-prime-monophosphate (IMP) and other purine nucleotides, and is allosterically activated by various compounds, including ATP. The enzyme is exclusively located in the cytoplasmic matrix of cells and may have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases.
• Source: E.Coli.
• Sequence: 1-561.
• Purity: ~95%.
• Specific Activity: n/aH.
• Applications: This recombinant protein can be used for WB, ELISA, MS and neutralization assays.
• Buffer: 10 mM Tris, pH 8.0, 0.1% Triton X-100, 0.002% NaN3, 10mM DTT.
• Storage: Store at -70?C. As with any protein, exposing NT5C2 recombinant protein to repeated freeze/thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
• SDS-PAGE: SDS PAGE Analysis of NT5C2 Recombinant Protein. 4-20% SDS gradient gel. Coomassie blue staining.

Gene Information

• Gene Name: NT5C2 5"-nucleotidase, cytosolic II [ Homo sapiens ]
• Synonyms: NT5C2; 5"-nucleotidase, cytosolic II; GMP; NT5B; PNT5; cN-II; IMP-specific 5"-NT; purine 5" nucleotidase; 5"-nucleotidase (purine), cytosolic type B; EC 3.1.3.5
• Gene ID: 22978
• mRNA Refseq: NM_001134373
• Protein Refseq: NP_001127845
• MIM: 600417
• UniProt ID: P49902
• Chromosome Location: 10q24.32-q24.33
• Pathway: Metabolic pathways; Nicotinate and nicotinamide metabolism; Purine metabolism; Pyrimidine metabolism; Metablism of nucleotides
• Function: 5"-nucleotidase activity; hydrolase activity; magnesium ion binding; nucleotide binding; protein binding