Recombinant Human POLE3, His-tagged

• Cat.No.: POLE3-397H
• Product Overview: Recombinant Human POLE3 was expressed inE.Coliusing an N-terminal His tag. MW=16.9 kDa.

Specification

• Cat. No.: POLE3-397H
• Description: POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.
• Source: E.Coli.
• Sequence: 1-147.
• Purity: ~95%.
• Specific Activity: n/aH.
• Applications: This recombinant protein can be used for WB, ELISA, MS and neutralization assays.
• Buffer: 10 mM Tris, pH 8.0, 0.002% NaN3, 3mM NaCl, 2.5mM.
• Storage: Store at -70˚C. As with any protein, exposing POLE3 recombinant protein to repeated freeze/thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
• SDS-PAGE: SDS PAGE Analysis of POLE3 Recombinant Protein. 4-20% SDS gradient gel. Coomassie blue staining.

Gene Information

• Gene Name: POLE3 polymerase (DNA directed), epsilon 3 (p17 subunit) [ Homo sapiens ]
• Synonyms: POLE3; polymerase (DNA directed), epsilon 3 (p17 subunit); DNA polymerase epsilon p17 subunit; DNA-directed DNA polymerase epsilon 3; arsenic transactivated protein; chromatin accessibility complex 17; histone fold protein CHRAC17; p17; YBL1; CHRAC17; CHARAC17
• Gene ID: 54107
• mRNA Refseq: NM_017443
• Protein Refseq: NP_059139
• MIM: 607267
• UniProt ID: Q9NRF9
• Chromosome Location: 9q33
• Pathway: Base excision repair; DNA replication; Metabolic pathways; Nucleotide excision repair; Purine metabolism; Pyrimidine metabolism
• Function: DNA-directed DNA polymerase activity; nucleotidyltransferase activity; protein binding; sequence-specific DNA binding; transferase activity