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Recombinant Human ARAF

Cat.No. : ARAF-26034TH
Product Overview : Recombinant fragment, corresponding to amino acids 282-606 of Human A RAF, containing the mutations YY301-302DD, with an N-terminal proprietary tag, expressed in a Baculovirus infected Sf9 cell expression system, 63.6 kDa.
  • Specification
  • Gene Information
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Description : This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development.
Tissue specificity : Predominantly in urogenital tissues.
Biological activity : Specific Activity: 50 nM A RAF yields 8% conversion of 1 uM ATP using MEK1 (K97R) as substrate.
Form : Liquid
Storage buffer : Preservative: NoneConstituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0
Storage : Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
Sequence Similarities : Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.Contains 1 phorbol-ester/DAG-type zinc finger.Contains 1 protein kinase domain.Contains 1 RBD (Ras-binding) domain.
Gene Name : ARAF v-raf murine sarcoma 3611 viral oncogene homolog [ Homo sapiens ]
Official Symbol : ARAF
Synonyms : ARAF; v-raf murine sarcoma 3611 viral oncogene homolog; ARAF1, v raf murine sarcoma 3611 viral oncogene homolog 1; serine/threonine-protein kinase A-Raf;
Gene ID : 369
mRNA Refseq : NM_001654
Protein Refseq : NP_001645
MIM : 311010
Uniprot ID : P10398
Chromosome Location : Xp11.3-p11.23
Pathway : Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem; Chronic myeloid leukemia, organism-specific biosystem;
Function : ATP binding; metal ion binding; nucleotide binding; protein binding; protein kinase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (24)

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Are there any specific cancer types where dysregulation of the ARAF protein is commonly observed? 02/19/2023

Dysregulation of the ARAF protein is predominantly found in certain cancer types, including melanoma, colorectal cancer, lung cancer, and thyroid cancer. Mutations or alterations in the ARAF gene can occur, resulting in its dysregulated activity and contributing to the development and progression of these cancers.

Are there any specific cancer types where dysregulation of the ARAF protein is commonly observed? 02/19/2023

Dysregulation of the ARAF protein is predominantly found in certain cancer types, including melanoma, colorectal cancer, lung cancer, and thyroid cancer. Mutations or alterations in the ARAF gene can occur, resulting in its dysregulated activity and contributing to the development and progression of these cancers.

Are there any known drugs that target the ARAF protein? 02/14/2023

Yes, several small molecule inhibitors that target the ARAF protein have been developed, such as sorafenib and RAF265. These drugs are primarily used in targeted cancer therapies and are designed to inhibit abnormal ARAF signaling in cancer cells.

Is the ARAF protein known to have any non-cancer-related functions? 10/04/2022

Although the ARAF protein is primarily studied in the context of cancer and signaling pathways, it is also believed to have non-cancer-related functions. It may be involved in processes like neuronal development, cardiac function, and immune responses. Further research is needed to fully understand the breadth of its functions.

Is the ARAF protein known to have any non-cancer-related functions? 10/04/2022

Although the ARAF protein is primarily studied in the context of cancer and signaling pathways, it is also believed to have non-cancer-related functions. It may be involved in processes like neuronal development, cardiac function, and immune responses. Further research is needed to fully understand the breadth of its functions.

Can the ARAF protein be targeted for drug development? 08/01/2021

Yes, the ARAF protein can be targeted for drug development. Small molecule inhibitors that specifically block the kinase activity of ARAF have been developed and studied as potential anti-cancer agents. These inhibitors aim to selectively inhibit abnormal ARAF signaling in cancer cells, while sparing normal cells.

How does the ARAF protein interact with other proteins in the MAPK/ERK pathway? 07/01/2021

The ARAF protein interacts with other proteins in the MAPK/ERK pathway to transmit signals and activate downstream effectors. It can form complexes with upstream proteins, such as growth factor receptors, to initiate signaling cascades. Additionally, it can interact with other kinases, scaffolding proteins, and adaptors to coordinate and amplify the signaling events.

How does the ARAF protein interact with other proteins in the MAPK/ERK pathway? 07/01/2021

The ARAF protein interacts with other proteins in the MAPK/ERK pathway to transmit signals and activate downstream effectors. It can form complexes with upstream proteins, such as growth factor receptors, to initiate signaling cascades. Additionally, it can interact with other kinases, scaffolding proteins, and adaptors to coordinate and amplify the signaling events.

How can research on the ARAF protein contribute to the development of novel therapeutic strategies? 12/24/2018

Research on the ARAF protein can provide insights into its role in cancer development and signaling pathways. By understanding the molecular mechanisms of ARAF activation and its interactions with other proteins, novel therapeutic strategies can be developed. This knowledge can inform the design of more effective ARAF inhibitors, combination therapies, or alternative approaches to target ARAF dysregulation in cancer cells.

How can research on the ARAF protein contribute to the development of novel therapeutic strategies? 12/24/2018

Research on the ARAF protein can provide insights into its role in cancer development and signaling pathways. By understanding the molecular mechanisms of ARAF activation and its interactions with other proteins, novel therapeutic strategies can be developed. This knowledge can inform the design of more effective ARAF inhibitors, combination therapies, or alternative approaches to target ARAF dysregulation in cancer cells.

How is the ARAF protein implicated in cancer development? 04/21/2018

The ARAF protein can contribute to cancer development through different mechanisms. Activating mutations in the ARAF gene can lead to aberrant activation of the MAPK/ERK signaling pathway, promoting uncontrolled cell growth, survival, and metastasis. Additionally, dysregulation of ARAF signaling can affect other cellular processes and contribute to tumor progression.

Does the ARAF protein have any role in normal development or tissue homeostasis? 03/04/2018

Yes, the ARAF protein plays a role in normal cellular processes and tissue homeostasis. It is involved in various developmental pathways and tissue-specific signaling cascades, contributing to normal growth, differentiation, and maintenance of tissues and organs.

Are there any ongoing clinical trials targeting the ARAF protein? 09/24/2017

There are ongoing clinical trials investigating the effectiveness and safety of ARAF inhibitors in cancer treatment. These trials are evaluating the potential of ARAF inhibitors as targeted therapies in specific cancer types and patient populations.

Are there any known diseases associated with mutations in the ARAF gene? 06/26/2017

Yes, mutations in the ARAF gene have been identified in some rare genetic disorders. For example, germline ARAF mutations have been linked to Noonan syndrome, a genetic condition characterized by abnormal facial features, heart defects, short stature, and developmental delays.

Are there any ongoing clinical trials targeting the ARAF protein for cancer treatment? 06/01/2017

Currently, there are no clinical trials specifically targeting the ARAF protein for cancer treatment listed on clinical trial databases like ClinicalTrials.gov. However, there may be preclinical studies or ongoing research investigating the potential of targeting ARAF in cancer therapy.

Are there any ongoing clinical trials targeting the ARAF protein for cancer treatment? 06/01/2017

Currently, there are no clinical trials specifically targeting the ARAF protein for cancer treatment listed on clinical trial databases like ClinicalTrials.gov. However, there may be preclinical studies or ongoing research investigating the potential of targeting ARAF in cancer therapy.

How common are ARAF mutations in cancer patients? 04/28/2017

ARAF mutations are relatively rare compared to other genes in the MAP kinase pathway, such as BRAF or KRAS. The frequency of ARAF mutations in cancer patients varies depending on the cancer type. For example, ARAF mutations are found in approximately 1-2% of melanomas and less frequently in other cancer types.

How common are ARAF mutations in cancer patients? 04/28/2017

ARAF mutations are relatively rare compared to other genes in the MAP kinase pathway, such as BRAF or KRAS. The frequency of ARAF mutations in cancer patients varies depending on the cancer type. For example, ARAF mutations are found in approximately 1-2% of melanomas and less frequently in other cancer types.

Are there any known side effects or limitations associated with targeting the ARAF protein for therapy? 01/07/2017

Targeting the ARAF protein for therapy can have potential side effects and limitations. Since ARAF is involved in various cellular processes, inhibiting its activity can impact normal cell functions and lead to adverse effects. Additionally, resistance mechanisms can arise, rendering ARAF inhibitors less effective over time. Careful monitoring and combination therapies may be necessary to minimize side effects and overcome resistance.

Are there any known side effects or limitations associated with targeting the ARAF protein for therapy? 01/07/2017

Targeting the ARAF protein for therapy can have potential side effects and limitations. Since ARAF is involved in various cellular processes, inhibiting its activity can impact normal cell functions and lead to adverse effects. Additionally, resistance mechanisms can arise, rendering ARAF inhibitors less effective over time. Careful monitoring and combination therapies may be necessary to minimize side effects and overcome resistance.

What are the potential clinical applications of targeting the ARAF protein? 12/16/2016

Targeting the ARAF protein could have potential clinical applications in cancer therapy. Mutations or dysregulation of the ARAF gene have been found in certain cancers, and inhibiting ARAF activity with specific inhibitors may help control tumor growth or sensitize cancer cells to other treatment modalities.

Can the ARAF protein be targeted for personalized medicine approaches? 11/28/2016

Yes, the ARAF protein can be targeted in personalized medicine approaches. By identifying specific mutations or dysregulation in the ARAF gene, treatment strategies can be tailored to individual patients. This allows for the selection of therapeutic options that specifically target the abnormal ARAF signaling in each patient's cancer, potentially improving treatment outcomes.

Can the ARAF protein be targeted for personalized medicine approaches? 11/28/2016

Yes, the ARAF protein can be targeted in personalized medicine approaches. By identifying specific mutations or dysregulation in the ARAF gene, treatment strategies can be tailored to individual patients. This allows for the selection of therapeutic options that specifically target the abnormal ARAF signaling in each patient's cancer, potentially improving treatment outcomes.

Can the ARAF protein be used as a biomarker for certain diseases? 10/14/2016

The ARAF protein itself is not commonly used as a biomarker for disease diagnosis or prognosis. However, the presence or activity of ARAF, along with other components of the MAPK/ERK signaling pathway, may be assessed in certain cancer types to guide treatment decisions or assess treatment response.

Customer Reviews (8)

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Reviews
01/17/2023

    Its unique characteristics contribute to obtaining clear and detailed structural information, enabling researchers to unravel the intricate complexities of protein interactions and conformational changes.

    08/17/2022

      The high-quality ARAF protein available from reliable manufacturers ensures accurate detection and quantification of ANO4 protein levels in samples, providing valuable insights into its biological roles and signaling pathways.

      05/24/2022

        In addition to their expertise, the manufacturer's technical support team can assist in troubleshooting and optimizing the handling and application of the ARAF protein.

        10/28/2021

          Its reliable and accurate performance ensures dependable results, contributing to the advancement of scientific knowledge in various disciplines.

          10/26/2020

            In WB experiments, ARAF protein exhibits exceptional performance, making it an ideal tool for studying ANO4 protein expression, regulation, and potential functional changes.

            09/04/2020

              ARAF protein demonstrates great utility in protein electron microscopy structure analysis.

              04/19/2020

                By relying on the ARAF protein and the manufacturer's exceptional technical support, researchers can confidently pursue their experimental goals while knowing they have access to reliable resources and expert guidance.

                04/15/2016

                  The use of ARAF protein in this technique allows researchers to explore the intricate three-dimensional structure of the protein at a molecular level.

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