Recombinant Human ARFIP2, His-tagged

• Cat.No.: ARFIP2-27057TH
• Product Overview: Recombinant fragment, corresponding to amino acids 1-261 of Human ARFIP2 with an N-terminal His Tag, approximately 30kDa

Specification

• Description: ARFIP2 is a ubiquitously expressed protein implicated in mediating cross talk between RAC and ARF small GTPases. It has been shown that ARFIP2 binds specifically to GTP-bound ARF1 and ARF6, but binds to Rac-GTP and Rac-GDP with similar affinities. The X-ray structure of arfaptin reveals an elongated, crescent-shaped dimer of 3-helix coiled-coils. Structures of arfaptin with Rac bound to either GDP or the slowly hydrolysable analog GMPPNP show that the switch regions adopt similar conformations in both complexes.
• Conjugation: HIS
• Source: E. coli
• Form: Lyophilised:reconstitution with 105 μl aqua dest.
• Storage buffer: Preservative: NoneConstituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequences of amino acids: MTDGILGKAATMEIPIHGNGEARQLPEDDGLEQDLQQVMV SGPNLNETSIVSGGYGGSGDGLIPTGSGRHPSHSTTPS GPGDEVARGIAGEKFDIVKKWGINTYKCTKQLLSERFG RGSRTVDLELELQIELLRETKRKYESVLQLGRALTAHLYS LLQTQHALGDAFADLSQKSPELQEEFGYNAETQKLLCK NGETLLGAVNFFVSSINTLVTKTMEDTLMTVKQYEAAR LEYDAYRTDLEELSLGPRDAGTRGRLESA

Gene Information

• Gene Name: ARFIP2 ADP-ribosylation factor interacting protein 2 [ Homo sapiens ]
• Official Symbol: ARFIP2
• Synonyms: ARFIP2; ADP-ribosylation factor interacting protein 2; arfaptin-2; arfaptin 2; POR1
• Gene ID: 23647
• mRNA Refseq: NM_001242854
• Protein Refseq: NP_001229783
• MIM: 601638
• Uniprot ID: P53365
• Chromosome Location: 11p15
• Pathway: Arf1 pathway, organism-specific biosystem
• Function: GTP binding; GTP-dependent protein binding; GTP-dependent protein binding; Rac GTPase binding; protein binding