Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-097-1828   Email: info@creative-biomart.com
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Native Human BCHE

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Cat.No.:
BCHE-26067TH
Product Overview:
Full length native Human Butyrylcholinesterase from Human plasma/serum. Amino acids 29-602, Predicted MWt 68kDa.
Description:
Mutant alleles at the BCHE locus are responsible for suxamethonium sensitivity. Homozygous persons sustain prolonged apnea after administration of the muscle relaxant suxamethonium in connection with surgical anesthesia. The activity of pseudocholinesterase in the serum is low and its substrate behavior is atypical. In the absence of the relaxant, the homozygote is at no known disadvantage.
Protein length:
574 amino acids
Molecular Weight:
65.000kDa
Tissue specificity:
Detected in blood plasma (at protein level). Present in most cells except erythrocytes.
Biological activity:
Butyrylcholinesterase enzyme assay: One unit of Butyrylcholinesterase will hydrolyse 1.0μ mole of Butyrylcholine per min at pH 8.0 at 37°C.
Form:
Lyophilised:Use of a phosphate buffer pH >7.0 containing 0.15M NaCl is recommended.
Storage buffer:
Preservative: NoneConstituents: 0.02M Ammonium bicarbonate. May contain traces of buffer salts.
Storage:
Store at +4°C.
Sequence Similarities:
Belongs to the type-B carboxylesterase/lipase family.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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