Recombinant Human Human CDK6, His-tagged

• Cat.No.: CCND1-31773TH
• Product Overview: Recombinant full length human CDK6 with N terminanl His tag and CCND1 with N-terminal proprietary tag co-expressed in a Baculovirus infected Sf9 cell expression system.

Specification

• Description: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.
• Conjugation: HIS
• Biological activity: Specific activity of CDK6 + CCND1 was determined to be 9.3 nmol/min/mg as per activity assay protocol.
• Form: Liquid
• Storage buffer: Preservative: 150mM ImidazoleConstituents: 25% Glycerol, 50mM Sodium phosphate, 300mM Sodium chloride, 0.2mM DTT, 0.1mM PMSF, pH 7.0
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.

Gene Information

• Gene Name: CCND1 cyclin D1 [ Homo sapiens ]
• Official Symbol: CCND1
• Synonyms: CCND1; cyclin D1; BCL1, cyclin D1 (PRAD1: parathyroid adenomatosis 1) , D11S287E, PRAD1; G1/S-specific cyclin-D1; B cell CLL/lymphoma 1; G1/S specific cyclin D1; parathyroid adenomatosis 1; U21B31
• Gene ID: 595
• mRNA Refseq: NM_053056
• Protein Refseq: NP_444284
• MIM: 168461
• Uniprot ID: P24385
• Chromosome Location: 11q13
• Pathway: ATF-2 transcription factor network, organism-specific biosystem; Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Bladder cancer, organism-specific biosystem
• Function: cyclin-dependent protein kinase regulator activity; enzyme binding; protein binding; protein kinase activity; protein kinase binding