"CDK9-CCNK" Related Products

Recombinant Human CDK9/CCNK Active protein, GST-tagged

Cat.No.: CDK9-26809TH
Product Overview: Recombinant full-length human CDK9 and CCNK were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.
Description: CDK9/CCNK is a member of the cyclin-dependent protein kinase (CDK) family. CDK9 is closely related to cdc28 and cdc2 and is an important regulator of the cell cycle. CDK9 is a component of the multiprotein complex TAK/P-TEFβ. CDK9 can modulate RNA polymerase II-directed transcription by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. CDK9 forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. CDK9 also interacts with the HIV-1 Tat protein which suggested a possible involvement of this protein in AIDS.
Source: Insect cell
Species: Human
Tag : GST
Form: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Bio-activity: 18nmol/min/mg
Molecular Mass: CDK9~68 kDa; CCNK~67 kDa
Purity: The purity of CDK9/CCNK was determined to be >85% by densitometry.
Applications: Kinase Assay; WB
Stability: 1 year at -70 centigrade
Storage: Store product at -70 centigrade
Concentration: 0.1 μg/μL
Gene Name: cyclin dependent kinase 9 [ Homo sapiens (human) ]
Official Symbol: CDK9
Synonyms: TAK; C-2k; CTK1; CDC2L4; PITALRE; CPR4
Gene ID: 1025
mRNA Refseq: NM_001261
Protein Refseq: NP_001252
MIM: 603251
UniProt ID: P50750

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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