Recombinant Human Twisted Gastrulation Homolog 1 (Drosophila)

• Cat.No.: TWSG1-1506H
• Product Overview: Recombinant human TSG is a 199 amino acid protein containing the BMP/TGFβ binding portion of the full length TSG protein expressed inE. colicells. MW=22.2 kDa.

Specification

• Cat. No.: TWSG1-1506H
• Description: Twisted Gastrulation Protein (TSG) is a secreted BMP binding protein structurally related to the BMP antagonists Chordin and Noggin. TSG can inhibit BMP activity by binding directly to BMP proteins, and can act either as a BMP4 agonist or antagonist (depending on the specific biochemical environment) by binding to the BMP4/Chordin complex.
• Source: E. coli.
• Quantity: 50 μg.
• Purity: Greater than 98% (determined by SDS-PAGE and HPLC analysis).
• Endotoxin Level: < 0.1 ng per μg of rHu TSG (1EU/μg).
• Biological Activity: Determined by its ability to neutralize BMP-6 induced alkaline phosphatase production by chondrogenic cells. The expected ED50 for this effect is < 2.0 μg/ml of TSG.
• Formulation: Lyophilized from a sterile-filtered solution with no additives.
• Reconstitution: Please Note: Always centrifuge product before opening vial. The lyophilized protein should be reconstituted with distilled, sterile water to a concentration of 0.1-1.0 mg/ml. For further dilution, carrier protein (0.1% BSA or HSA) should be added to avoid loss of bioactivity.
• Storage And Stability: The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: TWSG1 twisted gastrulation homolog 1 (Drosophila) [ Homo sapiens ]
• Synonyms: TWSG1; twisted gastrulation homolog 1 (Drosophila); TSG; Twisted gastrulation protein homolog 1; PSEC0250
• Gene ID: 57045
• mRNA Refseq: NM_020648
• Protein Refseq: NP_065699
• UniProt ID: Q9GZX9
• Chromosome Location: 18p11.3
• MIM: 605049
• Function: protein binding