Active Recombinant Human CIRBP protein, His-tagged

• Cat.No.: CIRBP-27194TH
• Product Overview: Recombinant Human CIRBP protein(NP_001271.1)(Met1~Glu172), fused to N-terminal His Tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Bio-activity: CIRBP (Cold-inducible RNA-binding protein) is considered to play a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival and act as a translational activator. Besides, ATXN1 (Ataxin-1) has been proven as an interactor of CIRBP. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human CIRBP and recombinant human ATXN1. Briefly, CIRBP were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ATXN1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CIRBP mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of CIRBP and ATXN1 was shown in Figure 1, and this effect was in a dose dependent manner.
• Molecular Mass: 22/27kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Met1~Glu172
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles.
  Store at 2-8°C for one month.
  Aliquot and store at -80ºC for 12 months.
• Concentration: 400µg/mL
• Reconstitution: Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: CIRBP cold inducible RNA binding protein [ Homo sapiens ]
• Official Symbol: CIRBP
• Synonyms: CIRP; A18HNRNP
• Gene ID: 1153
• mRNA Refseq: NM_001280.2
• Protein Refseq: NP_001271.1
• MIM: 602649
• UniProt ID: Q14011