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Recombinant Human Glutaryl-CoA Dehydrogenase, His-tagged

Cat.No. : GCDH-2728H
Product Overview : Recombinant human GCDH protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 45.8kDa (415aa),confirmed by MALDI-TOF.
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Cat. No. : GCDH-2728H
Description : Glutaryl-CoA dehydrogenase(GCDH) belongs to the acyl-CoA dehydrogenase family. GCDH catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO (2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. This Protein uses electron transfer flavoprotein as its electron acceptor. The enzyme exists in the mitochondrial matrix as a homotetramer of 45-kD subunits. Alternatively spliced transcript variants encoding different isoforms have been identified.
Source : E.coli.
Sequences Of Amino Acids : MGSSHHHHHH SSGLVPRGSH MRPEFDWQDP LVLEEQLTTD EILIRDTFRT YCQERLMPRI LLANRNEVFH REIISEMGEL GVLGPTIKGY GCAGVSSVAY GLLARELERV DSGYRSAMSV QSSLVMHPIY AYGSEEQRQK YLPQLAKGEL LGCFGLTEPN SGSDPSSMET RAHYNSSNKS YTLNGTKTWI TNSPMADLFV VWARCEDGCI RGFLLEKGMR GLSAPRIQGK FSLRASATGM IIMDGVEVPE ENVLPGASSL GGPFGCLNNA RYGIAWGVLG ASEFCLHTAR QYALDRMQFG VPLARNQLIQ KKLADMLTEI TLGLHACLQL GRLKDQDKAA PEMVSLLKRN NCGKALDIAR QARDMLGGNG ISDEYHVIRH AMNLEAVNTY EGTHDIHALI LGRAITGIQA FTASK
Form : Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 5mM DTT, 200mM NaCl.
Purity : > 90% by SDS – PAGE.
Concentration : 0.5 mg/ml (determined by Bradford assay).
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name : GCDH glutaryl-CoA dehydrogenase [ Homo sapiens ]
Synonyms : GCDH; glutaryl-CoA dehydrogenase; GCD; ACAD5; glutaryl-CoA dehydrogenase, mitochondrial; glutaryl-Coenzyme A dehydrogenase; EC 1.3.99.7
Gene ID : 2639
mRNA Refseq : NM_000159
Protein Refseq : NP_000150
MIM : 608801
UniProt ID : Q92947
Chromosome Location : 19p13.2
Pathway : Fatty acid metabolism; Lysine degradation; Metabolic pathways; Tryptophan metabolism
Function : FAD binding; glutaryl-CoA dehydrogenase activity; protein binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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