Recombinant Human DGKD, His-tagged

• Cat.No.: DGKD-26772TH
• Product Overview: Recombinant fragment, corresponding to amino acids 928-1170 of Human DGKD with a N terminal His tag; Predicted MWt 28 kDa:

Specification

• Description: This gene encodes a cytoplasmic enzyme that phosphorylates diacylglycerol to produce phosphatidic acid. Diacylglycerol and phosphatidic acid are two lipids that act as second messengers in signaling cascades. Their cellular concentrations are regulated by the encoded protein, and so it is thought to play an important role in cellular signal transduction. Alternative splicing results in two transcript variants encoding different isoforms.
• Conjugation: HIS
• Source: E. coli
• Form: Lyophilised:Reconstitution with 71 μl aqua dest.
• Storage buffer: Preservative: NoneConstituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequences of amino acids: MLSEEEATQMDQFGQAAGVLIHSIREIAQSHRDMEQELAH AVNASSKSMDRVYGKPRTTEGLNCSFVLEMVNNFRALR SETELLLSGKMALQLDPPQKEQLGSALAEMDRQLRRLA DTPWLCQSAEPGDEESVMLDLAKRSRSGKFRLVTKFKKEK NNKNKEAHSSLGAPVHLWGTEEVAAWLEHLSLCEYKDI FTRHDIRGSELLHLERRDLKDLGVTKVGHMKRILCGIK ELSRSAPAVEA

Gene Information

• Gene Name: DGKD diacylglycerol kinase, delta 130kDa [ Homo sapiens ]
• Official Symbol: DGKD
• Synonyms: DGKD; diacylglycerol kinase, delta 130kDa; diacylglycerol kinase, delta (130kD); diacylglycerol kinase delta; DGKdelta; diglyceride kinase; KIAA0145
• Gene ID: 8527
• mRNA Refseq: NM_003648
• Protein Refseq: NP_003639
• MIM: 601826
• Uniprot ID: Q16760
• Chromosome Location: 2q37
• Pathway: Effects of PIP2 hydrolysis, organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; Glycerolipid metabolism, organism-specific biosystem; Glycerolipid metabolism, conserved biosystem
• Function: ATP binding; diacylglycerol binding; diacylglycerol kinase activity; diacylglycerol kinase activity; metal ion binding