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Recombinant Murine Tumor Necrosis Factor

Cat.No. : Tnf-331M
Product Overview : Recombinant Murine Tumor Necrosis Factor-alpha (rMu TNF-alpha) is a pleiotrophic proinflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts expressed inSteptomyces lividans. MW=17.3kDa.
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Cat. No. : Tnf-331M
Description : TNF-alpha is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-alpha also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes. Recombinant murine TNF-alpha is a soluble 156 amino acid protein which corresponds to C-terminal extracellular domain of the full length transmembrane protein.
Source : Steptomyces lividans.
Quantity : 20 μg.
Specific Activity : ≥ 9 x 10⁸ IU/mg.
Purity : Greater than 98% (determined by SDS-PAGE and HPLC analysis).
Endotoxin Level : < 0.1 ng per μg of rMu TNF-alpha (1EU/μg).
Biological Activity : Recombinant human TNF-alpha is fully biologically active when compared to a standard. The ED₅₀, as determined by the cytolysis of murine L929 cells in the presence of actinomycin D, is 0.05 ng/ml corresponding to a specific activity of ≥ 9 x 10⁸ units/mg.
Formulation : Sterile, lyophilized protein. Each mg contains 50 mM Tris-HCl (pH 8.0) and 20 mM NaCl.
Reconstitution : Please note: Always centrifuge product briefly before opening the vial. The lyophilized rMu TNFalpha should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be further diluted into other aqueous buffers and stored at -20°C for future use.
Storage And Stability : The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rMu TNF-alpha protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Gene Name : Tnf tumor necrosis factor [ Mus musculus ]
Synonyms : Tnf; tumor necrosis factor; DIF; Tnfa; TNFSF2; Tnfsf1a; TNFalpha; MGC151434; TNF-alpha; TNF alpha; tumor necrosis factor alpha; tumor necrosis factor-alpha
Gene ID : 21926
mRNA Refseq : NM_013693
Protein Refseq : NP_038721
UniProt ID : P06804
Chromosome Location : 19.06 cM
Pathway : Adipocytokine signaling pathway; Allograft rejection; Alzheimer"s disease; Amyotrophic lateral sclerosis (ALS); Apoptosis; Asthma; Cytokine-cytokine receptor interaction; Fc epsilon RI signaling pathway; Graft-versus-host disease; Hematopoietic cell lineage; Hypertrophic cardiomyopathy (HCM); MAPK signaling pathway; Natural killer cell mediated cytotoxicity; RIG-I-like receptor signaling pathway; Systemic lupus erythematosus; T cell receptor signaling pathway; TGF-beta signaling pathway; Toll-like receptor signaling pathway; Type I diabetes mellitus; Type II diabetes mellitus
Function : cytokine activity; tumor necrosis factor receptor binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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