| Description : |
Cadherin 1, type 1, E-cadherin (epithelial), also known as CDH1 and CD32, is a tumor suppressor gene. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. E-cadherin-mediated cell-cell adhesion pathway may represent a novel chemotherapeutic target for bladder cancer, prostate cancer, and renal-cell carcinoma. |
| Source : |
Mammalian cells |
| Species : |
Mouse |
| Form : |
Lyophilized |
| Bio-activity : |
The biological activity of Mouse E-CAD was determined by the ability of the immobilized protein to support the adhesion of the MCF7 human breast adenocarcinoma cells. When 5 x 10^4 cells/well are added to rmECAD/Fc Chimera coated plates (1.5 μg/ml with 100 μl/well), approximately 50-70% will adhere after 90 minutes at room temperature.
Each investigator should determine their own optimal working dilution for specific applications. |
| Molecular Mass : |
The predicted molecular weight of Recombinant Mouse ECAD is Mr 88.2 kDa (monomer). However, the actual molecular weight as observed by migration on SDS Page is Mr 120 kDa. |
| AA Sequence : |
dwvippiscpenekgefpknlvqik snrdketkvfysitgqgadkppvgv fiieretgwlkvtqpldreaiakyi lyshavssngeavedpmeivitvtd qndnrpeftqevfegsvaegavpgt svmkvsatdadddvntynaaiayti vsqdpelphknmftvnrdtgvisvl tsgldresyptytlvvqaadlqgeg lsttakavitvkdindnapvfnpst yqgqvpenevnariatlkvtdddap ntpawkavytvvndpdqqfvvvtdp ttndgilktakgldfeakqqyilhv rveneepfegslvpstatvtvdvvd vneapifmpaerrvevpedfgvgqe itsytarepdtfmdqkityriwrdt anwleinpetgaiftraemdredae hvknstyvaliiatddgspiatgtg tlllvlldvndnapipeprnmqfcq rnpqphiitildpdlppntspftae lthgasvnwtieyndaaqeslilqp rkdleigeykihlkladnqnkdqvt tldvhvcdcegtvnncmkagivaag lqviegrmdpkscdkthtcppcpap ellggpsvflfppkpkdtlmisrtp evtcvvvdvshedpevkfnwyvdgv evhnaktkpreeqynstyrvvsvlt vlhqdwlngkeykckvsnkalpapi ektiskakgqprepqvytlppsrde ltknqvsltclvkgfypsdiavewe sngqpennykttppvldsdgsffly skltvdksrwqqgnvfscsvmheal hnhytqkslslspgkhhhhhh |
| Endotoxin : |
<1.0 EU/μg as determined by the LAL method |
| Purity : |
>90% by SDS-PAGE and analyzed by silver stain. |
| Notes : |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |
| Storage : |
This lyophilized protein is stable for six to twelve months when stored desiccated at -20 centigrade to -70 centigrade. After aseptic reconstitution, this protein may be stored at 2 centigrade to 8 centigrade for one month or at -20 centigrade to -70 centigrade in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. |
| Storage Buffer : |
This recombinant protein was 0.2 µm filtered and lyophilized from a sterile solution containing 50mM Tris-Citrate, 50 mM NaCl and 2 mM CaCl2, pH 6.5. |
