Recombinant Human Desmoglein 2

• Cat.No.: DSG2-1601H

Specification

• Cat. No.: DSG2-1601H
• Description: Desmoglein 2, also known as DSG2, is a human gene. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmoglein 2 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. It provides cellular adhesion and forces transduction by cell-to-cell anchorage. It also plays a role in embryonic stem cell proliferation.
• Molecular Weight: The predicted molecular weight of Recombinant Human Desmoglein-2 is Mr 89 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 115 kDa.
• Source: Mammalian cells
• Amino Acid Sequence: Human Desmoglein2 (Ala49 Gly608) - IEGRMD - Human IgG1 (Pro100 Lys330).
• State Of Matter: Lyophilized.
• Purity: >95% by SDS Page and analyzed by silver stain.
• Formulation: This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2-7.3 with no calcium, magnesium, or preservatives.
• Endotoxin: <1.0 EU/µg as determined by the LAL method.
• Biological Activity: The biological activity of Human DSG2 was determined by the ability of the immobilized protein to support the adhesion of BUD8 human skin fibroblast cells. When 3 x 104 cells are added to recombinant human Desmoglein 2 coated plates (10 μg/ml, 100 μl/well), approximately 50%-80% will adhere after 1 hour at 37°C.
• Storage And Stability: This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70℃. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
• Publication:
  An autoantibody identifies arrhythmogenic right ventricular cardiomyopathy and participates in its pathogenesis (2018)
  Catalytic antibodies in arrhythmogenic cardiomyopathy patients cleave desmoglein 2 and N-cadherin and impair cardiomyocyte cohesion (2023)
  Evaluation of autoantibodies to desmoglein-2 in dogs with and without cardiac disease (2023)
  Higher affinities of fibers with cell receptors increase the infection capacity and virulence of human adenovirus type 7 and type 55 compared to type 3 (2023)

Gene Information

• Gene Name: DSG2 desmoglein 2 [ Homo sapiens ]
• Synonyms: DSG2; desmoglein 2; HDGC; CDHF5; ARVC10; ARVD10; CMD1BB; MGC117034; MGC117036; MGC117037; Desmoglein-2
• Gene ID: 1829
• mRNA Refseq: NM_001943
• Protein Refseq: NP_001934
• MIM: 125671
• UniProt ID: Q14126
• Chromosome Location: 18q12.1
• Pathway: Arrhythmogenic right ventricular cardiomyopathy (ARVC)
• Function: calcium ion binding; protein binding