Recombinant Human Glutamate-ammonia Ligase, His-tagged

• Cat.No.: GLUL-769H
• Product Overview: Recombinant human GLUL protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 44.2kDa (393aa), confirmed by MALDI-TOF.

Specification

• Cat. No.: GLUL-769H
• Description: Glutamine synthetase (GLUL), which is therefore able to regulate intracellular concentrations of glutamate. GLUL catalyzes the synthesis of glutamine from glutamate and ammonia. Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling. GLUL is essential for proliferation of fetal skin fibroblasts and plays an important role in controlling body pH by removing ammonia from circulation. Mutations in GLUL are associated with congenital glutamine deficiency.
• Source: E.coli.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MTTSASSHLN KGIKQVYMSL PQGEKVQAMY IWIDGTGEGL RCKTRTLDSE PKCVEELPEW NFDGSSTLQS EGSNSDMYLV PAAMFRDPFR KDPNKLVLCE VFKYNRRPAE TNLRHTCKRI MDMVSNQHPW FGMEQEYTLM GTDGHPFGWP SNGFPGPQGP YYCGVGADRA YGRDIVEAHY RACLYAGVKI AGTNAEVMPA QWEFQIGPCE GISMGDHLWV ARFILHRVCE DFGVIATFDP KPIPGNWNGA GCHTNFSTKA MREENGLKYI EEAIEKLSKR HQYHIRAYDP KGGLDNARRL TGFHETSNIN DFSAGVANRS ASIRIPRTVG QEKKGYFEDR RPSANCDPFS VTEALIRTCL LNETGDEPFQ YKN
• Form: Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 5mM DTT, 200mM NaCl.
• Purity: > 90% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: GLUL glutamate-ammonia ligase [ Homo sapiens ]
• Synonyms: GLUL; glutamate-ammonia ligase; GS; GLNS; PIG43; PIG59; glutamine synthetase; glutamine synthase; glutamate decarboxylase; glutamate--ammonia ligase; proliferation-inducing protein 43; cell proliferation-inducing protein 59; EC 4.1.1.15; EC 6.3.1.2; glutamate-ammonia ligase (glutamine synthase)
• Gene ID: 2752
• mRNA Refseq: NM_001033044
• Protein Refseq: NP_001028216
• MIM: 138290
• UniProt ID: P15104
• Chromosome Location: 1q31
• Pathway: Alanine, aspartate and glutamate metabolism; Arginine and proline metabolism; Metabolic pathways; Nitrogen metabolism
• Function: ATP binding; glutamate decarboxylase activity; glutamate-ammonia ligase activity; identical protein binding; ligase activity; lyase activity; nucleotide binding