Recombinant Human IGF1R

• Cat.No.: IGF1R-29118TH
• Product Overview: Recombinant fragment, corresponding to amino acids 930-1337 of the cytoplasmic domain of Human IGF1 Receptor beta subunit fused with a proprietary tag; expressed in a baculovirus infected Sf9 cells, 72.3 kDa.

Specification

• Description: This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival.
• Biological activity: Specific activity: ~100 nmol/min/mg at 25°C using poly(Glu:Tyr)4:1 substrate.Reaction conditions are 50 mM Tris, pH 7.5, 5 mM MgCl2, 5 mM MnCl2, 1 mM DTT, 250 μM ATP. The kinase concentration was 0.5-1 μM and poly-L-lysine was added in equimolar amounts t
• Form: Liquid
• Storage buffer: Preservative: NoneConstituents: 50mM Tris, 1mM DTT, pH 7.5
• Storage: Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.

Gene Information

• Gene Name: IGF1R insulin-like growth factor 1 receptor [ Homo sapiens ]
• Official Symbol: IGF1R
• Synonyms: IGF1R; insulin-like growth factor 1 receptor; CD221; IGFIR; IGFR; JTK13; MGC18216
• Gene ID: 3480
• mRNA Refseq: NM_000875
• Protein Refseq: NP_000866
• MIM: 147370
• Uniprot ID: P08069
• Chromosome Location: 15q26.3
• Pathway: Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Apoptosis, organism-specific biosystem; Endochondral Ossification, organism-specific biosystem; Endocytosis, organism-specific biosystem
• Function: ATP binding; identical protein binding; insulin binding; insulin receptor binding; insulin receptor substrate binding