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Recombinant Human IL10, His-tagged

Cat.No. : IL10-28928TH
Product Overview : Recombinant fragment, corresponding to amino acids 19-178 of mature Human IL10 with an amino-terminal hexahistidine tag.
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Description : The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.
Conjugation : HIS
Source : E. coli
Tissue specificity : Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
Form : Liquid
Purity : >95% by SDS-PAGE
Storage buffer : Preservative: NoneConstituents: 50% Glycerol, 20mM Tris, pH 8
Storage : Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sequences of amino acids : SP GQGTQSENSC THFPGNLPNM LRDLRDAFSR VKTFFQMKDQLDNLLLKESL LEDFKGYLGC QALSEMIQFY LEEVMPQAEN QDPDIKAHVN SLGENLKTLRLRLRRCHRFL PCENKSKAVE QVKNAFNKLQ EKGIYKAMSE FDIFINYIEA YMTMKIRN
Sequence Similarities : Belongs to the IL-10 family.
Gene Name : IL10 interleukin 10 [ Homo sapiens ]
Official Symbol : IL10
Synonyms : IL10; interleukin 10; interleukin-10; CSIF; cytokine synthesis inhibitory factor; IL 10; IL10A; T cell growth inhibitory factor; TGIF;
Gene ID : 3586
mRNA Refseq : NM_000572
Protein Refseq : NP_000563
MIM : 124092
Uniprot ID : P22301
Chromosome Location : 1q31-q32
Pathway : African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Amoebiasis, organism-specific biosystem;
Function : cytokine activity; growth factor activity; interleukin-10 receptor binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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What state-of-the-art experimental strategies, including advanced imaging techniques, are implemented to meticulously assess the in vivo efficacy of IL-10-based therapies across a spectrum of inflammatory disorders? 02/12/2022

Cutting-edge imaging techniques, alongside in vivo disease models, allow for precise assessment of therapeutic effects and tissue responses to IL-10-based treatments.

How are advanced methods like single-cell RNA sequencing and flow cytometry harnessed to investigate the immunomodulatory impact of IL-10 across distinct immune cell subtypes? 02/10/2022

Advanced technologies, including single-cell RNA sequencing and flow cytometry, enable a granular analysis of IL-10's impact on diverse immune cell subpopulations.

Could you elaborate on the intricate experimental methodologies applied to unravel the influence of genetic polymorphisms within IL-10 and its receptor on disease susceptibility? 10/26/2021

Genotyping studies and genetic association analyses help correlate variations in IL-10 and its receptor with disease susceptibility across diverse populations.

How do researchers leverage advanced immunomonitoring tools to comprehensively evaluate the safety profiles of IL-10-based interventions in various clinical trial phases? 05/25/2021

Advanced immunomonitoring tools, including multiplex cytokine assays and immune cell phenotyping, provide comprehensive safety evaluations of IL-10 interventions in clinical trials.

How do researchers employ in vitro models to elucidate the mechanisms underlying IL-10's anti-inflammatory effects on immune cells? 12/03/2020

In vitro models involve exposing immune cells to IL-10 and assessing changes in cytokine secretion and cell activation markers to uncover its anti-inflammatory mechanisms.

What sophisticated experimental techniques are utilized to dissect the intricate signaling pathways downstream of IL-10 activation? 08/26/2020

State-of-the-art techniques such as phosphoproteomics, mass spectrometry, and protein-protein interaction assays provide insights into the intricate signaling cascades activated by IL-10.

Could you explain the multifaceted experimental strategies employed to determine the optimal dosage and administration routes for IL-10 in diverse animal models? 03/24/2019

Dose-response studies in animal models coupled with outcome measures like cytokine profiles and immune cell phenotypes help determine the optimal IL-10 dosage and administration routes.

What innovative experimental designs are employed to explore potential synergies between IL-10 and other cytokines, unraveling complex interplay in immune regulation? 11/23/2018

Experimental designs encompass co-stimulation experiments, coupled with functional assays and high-dimensional analysis, to elucidate synergistic effects involving IL-10 and other cytokines.

Can you elaborate on the comprehensive approaches scientists use to assess the tissue-specific expression patterns of IL-10 receptors in both normal and pathological conditions? 11/05/2018

Techniques like RT-PCR, immunohistochemistry, and spatial transcriptomics are employed to characterize IL-10 receptor expression patterns in specific tissues and disease contexts.

How are cutting-edge techniques such as CRISPR/Cas9 gene editing utilized to dissect the regulatory roles of IL-10 in complex autoimmune disease models? 02/22/2018

CRISPR/Cas9 gene editing is used to create knockout or overexpression models, combined with functional assays, to decipher IL-10's regulatory roles in autoimmune disease pathways.

Customer Reviews (3)

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Reviews
09/27/2022

    Effective in anti-inflammatory assays.

    01/09/2019

      High purity levels.

      04/15/2017

        Stable under long-term storage.

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