| Description : |
The protein encoded by this gene is a T cell-derived cytokine that shares sequence similarity with IL17. This cytokine was reported to stimulate the release of TNF alpha (TNF) and IL1 beta (IL1B) from a monocytic cell line. Immunohistochemical analysis of several nerve tissues indicated that this cytokine is primarily localized to neuronal cell bodies. Alternative splicing results in multiple splice variants. |
| Source : |
E.coli |
| Species : |
Human |
| Form : |
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4, with 0.1 % Tween-20 and 3 % Trehalose. |
| Bio-activity : |
Fully biologically active when compared to standard. The ED50 as determined by inducing IL-8 secretion of human HepG2 cells is less than 1.0 μg/ml, corresponding to a specific activity of > 1000 IU/mg. |
| Molecular Mass : |
Approximately 36.5 kDa, a non-disulfide-linked homodimer of two 161 amino acid polypeptide chains. |
| Protein length : |
161 |
| AA Sequence : |
MQPRSPKSKRKGQGRPGPLAPGPHQ VPLDLVSRMKPYARMEEYERNIEEM VAQLRNSSELAQRKCEVNLQLWMSN KRSLSPWGYSINHDPSRIPVDLPEA RCLCLGCVNPFTMQEDRSMVSVPVF SQVPVRRRLCPPPPRTGPCRQRAVM ETIAVGCTCIF |
| Endotoxin : |
Less than 0.1 EU/µg of rHuIL-17B as determined by LAL method. |
| Purity : |
>95% by SDS-PAGE and HPLC analysis. |
| Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
| Reconstitution : |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
