Recombinant Human MDM2, His-tagged
Cat.No. : | MDM2-29756TH |
Product Overview : | Recombinant full length human MDM2 with His tag; amino acids 1-491, approximately 56kDa. |
- Specification
- Gene Information
- Related Products
Description : | This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues. |
Conjugation : | HIS |
Source : | E. coli |
Tissue specificity : | Ubiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform Mdm2-G are observed in a range of cancers but absent in normal tissues. |
Biological activity : | 1 unit equals 1 nanogram of purified protein. |
Form : | Liquid |
Purity : | Immunogen affinity purified |
Storage buffer : | Preservative: NoneConstituents: 20% Glycerol, 20mM Tris Cl, 100mM Potassium chloride, 1mM DTT, 0.2mM EDTA, pH 8.0 |
Storage : | Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles. |
Sequence Similarities : | Belongs to the MDM2/MDM4 family.Contains 1 RanBP2-type zinc finger.Contains 1 RING-type zinc finger.Contains 1 SWIB domain. |
Gene Name : | MDM2 Mdm2 p53 binding protein homolog (mouse) [ Homo sapiens ] |
Official Symbol : | MDM2 |
Synonyms : | MDM2; Mdm2 p53 binding protein homolog (mouse); Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse) , mouse double minute 2, human homolog of; p53 binding protein; E3 ubiquitin-protein ligase Mdm2; HDM2; HDMX; MGC5370; |
Gene ID : | 4193 |
mRNA Refseq : | NM_002392 |
Protein Refseq : | NP_002383 |
Uniprot ID : | Q00987 |
Chromosome Location : | 12q13-q14 |
Pathway : | AKT phosphorylates targets in the cytosol, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Apoptosis, organism-specific biosystem; Aurora A signaling, organism-specific biosystem; Bladder cancer, organism-specific biosystem; |
Function : | enzyme binding; ligase activity; metal ion binding; p53 binding; protein binding; |
Products Types
◆ Recombinant Protein | ||
MDM2-0426H | Recombinant Human MDM2 Protein (S17-N111), Tag Free | +Inquiry |
MDM2-3449H | Recombinant Human MDM2 Protein, His (Fc)-Avi-tagged | +Inquiry |
Mdm2-1771M | Recombinant Mouse Mdm2 Protein, His-tagged | +Inquiry |
MDM2-651M | Recombinant Mouse MDM2 Protein (1-489 aa), His-tagged | +Inquiry |
MDM2-2814H | Recombinant Human MDM2 protein(31-260 aa), C-His-tagged | +Inquiry |
◆ Lysates | ||
MDM2-4404HCL | Recombinant Human MDM2 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionMDM2 binds directly to p53, inhibiting its transcriptional activity. Additionally, MDM2 ubiquitinates p53, targeting it for proteasomal degradation, thus regulating p53 levels in the cell.
The MDM2-p53 feedback loop is crucial for cellular homeostasis. When DNA damage occurs, p53 levels rise, leading to cell cycle arrest or apoptosis. Elevated p53 can induce MDM2 expression, which in turn downregulates p53, ensuring that p53 activity is transient.
Amplifications or overexpression of MDM2 can lead to reduced p53 activity, even in the presence of DNA damage or other stress signals, potentially contributing to uncontrolled cell proliferation and tumorigenesis.
Several small molecule inhibitors, like Nutlin-3 and RG7112, target MDM2 to prevent its interaction with p53, thereby stabilizing and activating p53. These inhibitors hold therapeutic potential, especially in tumors with wild-type p53 but overexpressed MDM2.
Besides p53, MDM2 interacts with other proteins, including E2F1 and RB, influencing cell cycle progression and apoptosis. The balance of these interactions can determine cell fate.
The MDM2 gene encodes a nuclear-localized E3 ubiquitin ligase. Its primary function is to regulate the p53 tumor suppressor protein's stability and activity.
Therapeutic strategies targeting MDM2 include the use of MDM2 inhibitors to reactivate p53, combination therapies with DNA-damaging agents, and strategies to disrupt MDM2's interaction with other proteins to modulate cell cycle and apoptosis.
Customer Reviews (3)
Write a reviewValidated in multiple assays.
Great for cell culture.
Consistent performance, highly reproducible.
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