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Recombinant Human MGMT protein, His-tagged

Cat.No. : MGMT-28449TH
Product Overview : Recombinant Human MGMT(1-207aa) fused with His tag at N-terminal was expressed in E. coli.
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  • Gene Information
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Description : O-6-methylguanine-DNA methyltransferase (MGMT) is an enzyme that repairs O-6-methylguanine, a mutagenic DNA base damaged by endogenous and environmental alkylating agents and is involved in the cellular defense against the biological effects of O-6-methylguanine in DNA. MGMT repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. There are few reports that abnormal MGMT expression correlates with the prognosis in human solid cancers.
Source : E. coli
Species : Human
Tag : His
Form : Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing 1 mM DTT, 10% glycerol
Molecular Mass : 23.8 kDa (227 aa), confirmed by MALDI-TOF. (Molecular weight on SDS-PAGE will appear higher)
AA Sequence : MGSSHHHHHH SSGLVPRGSH MDKDCEMKRT TLDSPLGKLE LSGCEQGLHE IKLLGKGTSA ADAVEVPAPA AVLGGPEPLM QCTAWLNAYFHQPEAIEEFP VPAFHHPVFQ QESFTRQVLW KLLKVVKFGE VISYQQLAAL AGNPKAARAV GGAMRGNPVP ILIPCHRVVC SSGAVGNYSGGLAVKEWLLA HEGHRLGKPG LGGSSGLAGA WLKGAGATSG SPPAGRN
Endotoxin : < 1.0 EU per 1 microgram of protein (determined by LAL method)
Purity : > 95% by SDS - PAGE
Storage : Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
Concentration : 0.5 mg/ml (determined by Bradford assay)
Gene Name : MGMT O-6-methylguanine-DNA methyltransferase [ Homo sapiens ]
Official Symbol : MGMT
Synonyms : MGMT; O-6-methylguanine-DNA methyltransferase; methylated-DNA--protein-cysteine methyltransferase; methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase; O6-methylguanine-DNA methyltransferase; 6-O-methylguanine-DNA methyltransferase;
Gene ID : 4255
mRNA Refseq : NM_002412
Protein Refseq : NP_002403
MIM : 156569
UniProt ID : P16455
Chromosome Location : 10q26
Pathway : DNA Damage Reversal, organism-specific biosystem; DNA Repair, organism-specific biosystem;
Function : DNA binding; DNA-methyltransferase activity; calcium ion binding; methylated-DNA-[protein]-cysteine S-methyltransferase activity; methyltransferase activity; transferase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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Can MGMT status be used to predict the response to other types of cancer treatments? 01/31/2022

Yes, MGMT status has implications beyond glioblastoma and can influence the response to various alkylating agents in different cancer types.

Can the MGMT protein be targeted for cancer prevention strategies? 03/24/2020

While direct targeting is challenging, understanding MGMT's role may lead to the development of preventive strategies, such as identifying high-risk individuals for regular screenings.

Is MGMT involved in resistance mechanisms to chemotherapy? 10/08/2018

Yes, MGMT expression can confer resistance to alkylating agents, making it an important factor in understanding and overcoming chemotherapy resistance.

How does MGMT repair DNA damage caused by environmental factors like radiation? 08/21/2018

MGMT repairs DNA damage by directly removing alkyl groups from the O6-position of guanine, which can be induced by exposure to radiation and certain chemicals.

How does MGMT status affect the choice of chemotherapy in glioblastoma patients? 04/25/2018

Glioblastoma patients with MGMT promoter methylation are more likely to benefit from alkylating agents like temozolomide.

Customer Reviews (3)

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Reviews
05/31/2021

    The MGMT protein is an exceptional protein solution that exceeds expectations in terms of quality and suitability for my experimental requirements.

    12/15/2018

      In addition to its outstanding quality, the MGMT protein comes with exceptional technical support from its manufacturer.

      03/07/2017

        I am confident that utilizing the MGMT protein will empower me to uncover crucial insights into cellular mechanisms with precision.

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