Recombinant Human MGMT protein, His-tagged
Cat.No. : | MGMT-28449TH |
Product Overview : | Recombinant Human MGMT(1-207aa) fused with His tag at N-terminal was expressed in E. coli. |
- Specification
- Gene Information
- Related Products
Description : | O-6-methylguanine-DNA methyltransferase (MGMT) is an enzyme that repairs O-6-methylguanine, a mutagenic DNA base damaged by endogenous and environmental alkylating agents and is involved in the cellular defense against the biological effects of O-6-methylguanine in DNA. MGMT repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. There are few reports that abnormal MGMT ex |
Source : | E. coli |
Species : | Human |
Tag : | His |
Form : | Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing 1 mM DTT, 10% glycerol |
Molecular Mass : | 23.8 kDa (227 aa), confirmed by MALDI-TOF. (Molecular weight on SDS-PAGE will appear higher) |
AA Sequence : | MGSSHHHHHH SSGLVPRGSH MDKDCEMKRT TLDSPLGKLE LSGCEQGLHE IKLLGKGTSA ADAVEVPAPA AVLGGPEPLM QCTAWLNAYFHQPEAIEEFP VPAFHHPVFQ QESFTRQVLW KLLKVVKFGE VISYQQLAAL AGNPKAARAV GGAMRGNPVP ILIPCHRVVC SSGAVGNYSGGLAVKEWLLA HEGHRLGKPG LGGSSGLAGA WLKGAGATSG SPPAGRN |
Endotoxin : | < 1.0 EU per 1 microgram of protein (determined by LAL method) |
Purity : | > 95% by SDS - PAGE |
Storage : | Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles. |
Concentration : | 0.5 mg/ml (determined by Bradford assay) |
Gene Name : | MGMT O-6-methylguanine-DNA methyltransferase [ Homo sapiens ] |
Official Symbol : | MGMT |
Synonyms : | MGMT; O-6-methylguanine-DNA methyltransferase; methylated-DNA--protein-cysteine methyltransferase; methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase; O6-methylguanine-DNA methyltransferase; 6-O-methylguanine-DNA methyltransferase; |
Gene ID : | 4255 |
mRNA Refseq : | NM_002412 |
Protein Refseq : | NP_002403 |
MIM : | 156569 |
UniProt ID : | P16455 |
Chromosome Location : | 10q26 |
Pathway : | DNA Damage Reversal, organism-specific biosystem; DNA Repair, organism-specific biosystem; |
Function : | DNA binding; DNA-methyltransferase activity; calcium ion binding; methylated-DNA-[protein]-cysteine S-methyltransferase activity; methyltransferase activity; transferase activity; |
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◆ Lysates | ||
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
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Q&As (5)
Ask a questionYes, MGMT status has implications beyond glioblastoma and can influence the response to various alkylating agents in different cancer types.
While direct targeting is challenging, understanding MGMT's role may lead to the development of preventive strategies, such as identifying high-risk individuals for regular screenings.
Yes, MGMT expression can confer resistance to alkylating agents, making it an important factor in understanding and overcoming chemotherapy resistance.
MGMT repairs DNA damage by directly removing alkyl groups from the O6-position of guanine, which can be induced by exposure to radiation and certain chemicals.
Glioblastoma patients with MGMT promoter methylation are more likely to benefit from alkylating agents like temozolomide.
Customer Reviews (3)
Write a reviewThe MGMT protein is an exceptional protein solution that exceeds expectations in terms of quality and suitability for my experimental requirements.
In addition to its outstanding quality, the MGMT protein comes with exceptional technical support from its manufacturer.
I am confident that utilizing the MGMT protein will empower me to uncover crucial insights into cellular mechanisms with precision.
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