"MGMT" Related Products

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Recombinant Human MGMT protein, His-tagged

Cat.No.: MGMT-28449TH
Product Overview: Recombinant Human MGMT(1-207aa) fused with His tag at N-terminal was expressed in E. coli.
Description: O-6-methylguanine-DNA methyltransferase (MGMT) is an enzyme that repairs O-6-methylguanine, a mutagenic DNA base damaged by endogenous and environmental alkylating agents and is involved in the cellular defense against the biological effects of O-6-methylguanine in DNA. MGMT repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. There are few reports that abnormal MGMT expression correlates with the prognosis in human solid cancers.
Source: E. coli
Species: Human
Tag: His
Form: Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing 1 mM DTT, 10% glycerol
Molecular Mass: 23.8 kDa (227 aa), confirmed by MALDI-TOF. (Molecular weight on SDS-PAGE will appear higher)
AA Sequence: MGSSHHHHHH SSGLVPRGSH MDKDCEMKRT TLDSPLGKLE LSGCEQGLHE IKLLGKGTSA ADAVEVPAPA AVLGGPEPLM QCTAWLNAYFHQPEAIEEFP VPAFHHPVFQ QESFTRQVLW KLLKVVKFGE VISYQQLAAL AGNPKAARAV GGAMRGNPVP ILIPCHRVVC SSGAVGNYSGGLAVKEWLLA HEGHRLGKPG LGGSSGLAGA WLKGAGATSG SPPAGRN
Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
Purity: > 95% by SDS - PAGE
Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
Concentration: 0.5 mg/ml (determined by Bradford assay)
Gene Name: MGMT O-6-methylguanine-DNA methyltransferase [ Homo sapiens ]
Official Symbol: MGMT
Synonyms: MGMT; O-6-methylguanine-DNA methyltransferase; methylated-DNA--protein-cysteine methyltransferase; methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase; O6-methylguanine-DNA methyltransferase; 6-O-methylguanine-DNA methyltransferase;
Gene ID: 4255
mRNA Refseq: NM_002412
Protein Refseq: NP_002403
MIM: 156569
UniProt ID: P16455
Chromosome Location: 10q26
Pathway: DNA Damage Reversal, organism-specific biosystem; DNA Repair, organism-specific biosystem;
Function: DNA binding; DNA-methyltransferase activity; calcium ion binding; methylated-DNA-[protein]-cysteine S-methyltransferase activity; methyltransferase activity; transferase activity;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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