Recombinant Human NEK3

• Cat.No.: NEK3-29620TH
• Product Overview: Recombinant full length Human NEK3 with N-terminal proprietary tag expressed in a Baculovirus infected Sf9 cell expression system; amino acids 1-506, 86kDa.

Specification

• Description: This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. Multiple transcript variants encoding different isoforms have been found for this gene.
• Biological activity: The Specific activity of NEK3-29620TH was determined to be 132 nmol/min/mg.
• Form: Liquid
• Storage buffer: Preservative: NoneConstituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequence Similarities: Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.Contains 1 protein kinase domain.

Gene Information

• Gene Name: NEK3 NIMA (never in mitosis gene a)-related kinase 3 [ Homo sapiens ]
• Official Symbol: NEK3
• Synonyms: NEK3; NIMA (never in mitosis gene a)-related kinase 3; serine/threonine-protein kinase Nek3; glycogen synthase A kinase; HSPK36; hydroxyalkyl protein kinase; MGC29949; phosphorylase B kinase kinase; serine/threonine protein kinase NEK3
• Gene ID: 4752
• mRNA Refseq: NM_001146099
• Protein Refseq: NP_001139571
• MIM: 604044
• Uniprot ID: P51956
• Chromosome Location: 13q14.2-q21.1
• Function: ATP binding; ATP binding; metal ion binding; nucleotide binding; protein binding