Recombinant Human Selectin L, Fc-His

• Cat.No.: SELL-1660H
• Product Overview: Recombinant Human SELL was expressed inNSOcells. The extracellular domain of human L-Selectin (amino acid residues 1-332) was fused to the carboxy-terminal 6X his tagged Fc region of human IgG1 via a polypeptide linker.

Specification

• Cat. No.: SELL-1660H
• Description: L-selectin, also known as CD62L, is a cell adhesion molecule consisting of a large, highly glycosylated, extracellular domain found on leukocytes. It belongs to the selectin family of proteins, which recognise sialylated carbohydrate groups. L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via the high endothelial venules. L-selectin is an effective marker for separating lymphoid progenitors from myeloid progenitors and hematopoietic stem cells in bone marrow.
• Molecular Weight: The predicted molecular weight of Recombinant Human L-Selectin is Mr 60.4 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 90-100 kDa under reducing conditions.
• Source: Mammalian cells
• State Of Matter: Lyophilized.
• Formulation: This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
• Purity: >90% by SDS Page and analyzed by silver stain.
• Endotoxin: <1.0 EU/µg as determined by the LAL method.
• Biological Activity: The biological activity of Human L-Selectin was determined by the ability of the immobilized protein to support the adhesion of LS180 cells, a human colon adenocarcinoma cell line. When 1 x 104 cells/well are added to human L-Selectin coated plates (10 μg/ml with 100 μl/well), approximately 90 - 100% will adhere for 1 hour incubation at RT.
• Storage And Stability: This lyophilized protein is stable for six to twelve months when stored desiccated at -20℃ to -70℃. After aseptic reconstitution, this protein may be stored at 2℃ to 8℃ for one month or at -20℃ to -70℃ in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Gene Information

• Gene Name: SELL selectin L [ Homo sapiens ]
• Synonyms: SELL; selectin L; CD62L; LAM1; LECAM1; LEU8; LNHR; LSEL; LYAM1; PLNHR; TQ1; CD62 antigen-like family member L; L-selectin; gp90-MEL; leukocyte adhesion molecule-1; leukocyte surface antigen Leu-8; leukocyte-endothelial cell adhesion molecule 1; lymph node homing receptor; lymphocyte adhesion molecule 1; pln homing receptor; CD62L antigen; Leukocyte adhesion molecule 1; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; Lymph node homing receptor
• Gene ID: 6402
• mRNA Refseq: NM_000655
• Protein Refseq: NP_000646
• MIM: 153240
• UniProt ID: P14151
• Chromosome Location: 1q23-q25
• Pathway: Cell adhesion molecules (CAMs); Hemostasis; Signaling in Immune system
• Function: glycosphingolipid binding; heparin binding; protein binding; sugar binding