| Cat. No. : |
TNFSF8-1595H |
| Description : |
The CD30 ligand is a type II transmembrane glycoprotein of the tumor necrosis factor ligand superfamily. CD30L is expressed on the cell surface of activated T cells, B cells, and monocytes. Recombinant CD30L has been shown to co-stimulate T cells and to act as a mitogen for Hodgkin"s disease derived cell lines. The receptor for CD30L, CD30/ TNFRSF8/CD153, is a type I cytokine receptor that is highly expressed by activated T cells, Hodgkin and Reed- Sternberg (H-RS) cells, and anaplastic large cell lymphoma cells. CD30 ligation by CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation and cell death by apoptosis. |
| Molecular Weight : |
The predicted molecular weight of Recombinant Human CD30 Ligand is Mr 22 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 30-40 kDa. |
| Source : |
Mammalian cells |
| State Of Matter : |
Lyophilized. |
| Purity : |
>95% by SDS Page and analyzed by silver stain. |
| Formulation : |
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives. |
| Endotoxin : |
<1.0 EU/µg as determined by the LAL method. |
| Biological Activity : |
The biological activity of Human CD30L was determined by its ability to stimulate IL-6 secretion by HDLM cells, a Hodgkin's lymphoma cell line (Gruss, H-J. et al., 1996, Blood 87:2443). The expected ED50 for this effect is typically 0.1 - 0.4 μg/mL in the presence of 10 μg/mL of a cross-linking antibody Recombinant Rat Resistin + His-tag. |
| Storage And Stability : |
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions. |
