Recombinant Human PFKP, His-tagged

• Cat.No.: PFKP-30890TH
• Product Overview: Recombinant fragment, corresponding to amino acids 35-447 of Human PFKP with an N terminal His tag. Predicted mwt: 47 kDa

Specification

• Description: The PFKP gene encodes the platelet isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The PFKP gene, which maps to chromosome 10p, is also expressed in fibroblasts. See also the muscle (PFKM; MIM 610681) and liver (PFKL; MIM 171860) isoforms of phosphofructokinase, which map to chromosomes 12q13 and 21q22, respectively.
• Conjugation: HIS
• Source: E. coli
• Form: Lyophilised:Reconstitute with 91 μl aqua dest.
• Storage buffer: Preservative: NoneConstituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
• Storage: Store at 4°C. Upon reconstitution store at -80oC.
• Sequences of amino acids: DERRFQDAVRLRGRSFAGNLNTYKRLAIKLPDDQIPKTNC NVAVINVGAPAAGMNAAVRSAVRVGIADGHRMLAIYDG FDGFAKGQIKEIGWTDVGGWTGQGGSILGTKRVLPGKYLE EIATQMRTHSINALLIIGGFEAYLGLLELSAAREKHEE FCVPMVMVPATVSNNVPGSDFSIGADTALNTITDTCDR IKQSASGTKRRVFIIETMGGYCGYLANMGGLAAGADAAYI FEEPFDIRDLQSNVEHLTEKMKTTIQRGLVLRNESCSE NYTTDFIYQLYSEEGKGVFDCRKNVLGHMQQGGAPSPF DRNFGTKISARAMEWITAKLKEARGRGKKFTTDDSICVLG ISKRNVIFQPVAELKKQTDFEHRIPKEQWWLKLRPLMK ILAKYKASYDVSDSGQLEHVQPWSV
• Sequence Similarities: Belongs to the phosphofructokinase family. Two domains subfamily.

Gene Information

• Gene Name: PFKP phosphofructokinase, platelet [ Homo sapiens ]
• Official Symbol: PFKP
• Synonyms: PFKP; phosphofructokinase, platelet; 6-phosphofructokinase type C; PFK C; PFKF; Phosphofructokinase; platelet type
• Gene ID: 5214
• mRNA Refseq: NM_001242339
• Protein Refseq: NP_001229268
• MIM: 171840
• Uniprot ID: Q01813
• Chromosome Location: 10p15.3-p15.2
• Pathway: Fructose and mannose metabolism, organism-specific biosystem; Fructose and mannose metabolism, conserved biosystem; Galactose metabolism, organism-specific biosystem; Galactose metabolism, conserved biosystem; Glucose metabolism, organism-specific biosystem
• Function: 6-phosphofructokinase activity; 6-phosphofructokinase activity; 6-phosphofructokinase activity; ATP binding; fructose-6-phosphate binding