Recombinant Human POU5F1

• Cat.No.: POU5F1-28780TH
• Product Overview: Recombinant fragment, corresponding to amino acids 1-212 of Human Oct4; MWt 23kDa.

Specification

• Description: This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewings sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.
• Protein length: 212 amino acids
• Molecular Weight: 23.000kDa
• Source: E. coli
• Tissue specificity: Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 5% Glycerol, PBS (1.5% Monobasic dihydrogen potassium phosphate, 8.1mM Dibasic monohydrogen sodium phosphate, 500mM Sodium chloride, 2.7mM Potassium chloride, pH 7.4)
• Storage: Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
• Sequence Similarities: Belongs to the POU transcription factor family. Class-5 subfamily.Contains 1 homeobox DNA-binding domain.Contains 1 POU-specific domain.

Gene Information

• Gene Name: POU5F1 POU class 5 homeobox 1 [ Homo sapiens ]
• Official Symbol: POU5F1
• Synonyms: POU5F1; POU class 5 homeobox 1; OTF3, POU domain class 5, transcription factor 1; POU domain, class 5, transcription factor 1; MGC22487; OCT3; Oct4
• Gene ID: 5460
• mRNA Refseq: NM_001173531
• Protein Refseq: NP_001167002
• MIM: 164177
• Uniprot ID: Q01860
• Chromosome Location: 6p21.33
• Pathway: HIF-2-alpha transcription factor network, organism-specific biosystem; Wnt Signaling Pathway and Pluripotency, organism-specific biosystem
• Function: DNA binding; miRNA binding; protein binding; sequence-specific DNA binding; sequence-specific DNA binding RNA polymerase II transcription factor activity