Recombinant Human PTGS2, His-tagged

• Cat.No.: PTGS2-26360TH
• Product Overview: Recombinant full length COX2 / Cyclooxygenase 2 protein (Human), a homodimer (70-74 kDa/subunit), contains a six histidine tag near the N-terminus.Isolated from a Baculovirus overexpression system in Sf 21 cells.

Specification

• Description: Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis.
• Conjugation: HIS
• Biological activity: Specific Activity: minimum 8,000 units per mg protein
• Form: Liquid
• Storage buffer: Preservative: 300μM diethyldithio-carbamate (DDC)Constituents: 0.1% Tween 20, 80mM Tris HCl, pH 8Note: If this preservative (DDC) is undesirable, it can be removed by standard desalting procedures, but the enzyme is unstable in the absence of the preserva
• Storage: Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
• Sequence Similarities: Belongs to the prostaglandin G/H synthase family.Contains 1 EGF-like domain.

Gene Information

• Gene Name: PTGS2 prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) [ Homo sapiens ]
• Official Symbol: PTGS2
• Synonyms: PTGS2; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase); prostaglandin G/H synthase 2; COX2
• Gene ID: 5743
• mRNA Refseq: NM_000963
• Protein Refseq: NP_000954
• MIM: 600262
• Uniprot ID: P35354
• Chromosome Location: 1q25.2-q25.3
• Pathway: Arachidonic acid metabolism, organism-specific biosystem; Arachidonic acid metabolism, conserved biosystem; Biological oxidations, organism-specific biosystem; C-MYB transcription factor network, organism-specific biosystem; COX reactions, organism-specific biosystem
• Function: enzyme binding; heme binding; lipid binding; metal ion binding; oxidoreductase activity