Active Recombinant Mouse Flt1 Protein, Fc-tagged

• Cat.No.: Flt1-1785M
• Product Overview: Recombinant mouse Flt1 protein (Ser27-Glu759) with mouse IgG2A (Glu98-Lys330) tag at C-terminus was expressed in Mouse myeloma cell line.

Specification

• Description: Biased expression in placenta adult (RPKM 35.7), lung adult (RPKM 11.8) and 11 other tissues.
• Source: Mammalian cells
• Species: Mouse
• Tag: Fc
• Form: Lyophilized
• Bio-activity: Measured by its ability to inhibit the VEGF-dependent proliferation of HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 5-30 ng/mL.
• Molecular Mass: 109.6 kDa (monomer)
• Endotoxin: <0.10 EU per 1 µg of the protein by the LAL method.
• Purity: > 95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 µm filtered solution in PBS.
• Reconstitution: Reconstitute at 100 µg/mL in PBS.
• Shipping: The product is shipped at ambient temperature.
• Publication:
  Syndecan-1 and glycosaminoglycans in pre-eclamptic and normotensive pregnancies. (2017)
  Soluble syndecan-1 and glycosaminoglycans in preeclamptic and normotensive pregnancies (2021)
  Vascular damage and dysfunction in hypertensive emergencies (2017)
  SFlt-1 Elevates Blood Pressure by Augmenting Endothelin-1-Mediated Vasoconstriction in Mice (2014)

Gene Information

• Gene Name: Flt1 FMS-like tyrosine kinase 1 [ Mus musculus (house mouse) ]
• Official Symbol: Flt1
• Synonyms: Flt1; FMS-like tyrosine kinase 1; Flt-1; sFlt1; VEGFR1; VEGFR-1; AI323757; vascular endothelial growth factor receptor 1; embryonic receptor kinase 2; tyrosine-protein kinase receptor FLT; vascular permeability factor receptor; EC 2.7.10.1
• Gene ID: 14254
• mRNA Refseq: NM_010228
• Protein Refseq: NP_034358
• UniProt ID: P35969