Recombinant Human RANGAP1, His-tagged

• Cat.No.: RANGAP1-30474TH
• Product Overview: Recombinant full length protein, corresponding to amino acids 1-357 of Human RanGAP1 with N terminal His tag. MWt 41kDa

Specification

• Description: RanGAP1, is a homodimeric 65-kD polypeptide that specifically induces the GTPase activity of RAN, but not of RAS by over 1,000-fold. RanGAP1 is the immediate antagonist of RCC1, a regulator molecule that keeps RAN in the active, GTP-bound state. The RANGAP1 gene encodes a 587-amino acid polypeptide. The sequence is unrelated to that of GTPase activators for other RAS-related proteins, but is 88% identical to Fug1, the murine homolog of yeast Rna1p. RanGAP1 and RCC1 control RAN-dependent transport between the nucleus and cytoplasm. RanGAP1 is a key regulator of the RAN GTP/GDP cycle.
• Conjugation: HIS
• Source: E. coli
• Tissue specificity: Highly expressed in brain, thymus and testis.
• Form: Lyophilised:Reconstitution with 98 μl aqua dest.
• Storage buffer: Preservative: NoneConstituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequences of amino acids: MASEDIAKLAETLAKTQVAGGQLSFKGKSLKLNTAEDAKD VIKEIEDFDSLEALRLEGNTVGVEAARVIAKALEKKSE LKRCHWSDMFTGRLRTEIPPALISLGEGLITAGAQLVE LDLSDNAFGPDGVQGFEALLKSSACFTLQELKLNNCGMGI GGGKILAAALTECHRKSSAQGKPLALKVFVAGRNRLEN DGATALAEAFRVIGTLEEVHMPQNGINHPGITALAQAF AVNPLLRVINLNDNTFTEKGAVAMAETLKTLRQVEVINFG DCLVRSKGAVAIADAIRGGLPKLKELNLSFCEIKRDAA LAVAEAMADKAELEKLDLNGNTLGEEGCEQLQEVLEGF NMAKVLASL
• Sequence Similarities: Belongs to the RNA1 family.Contains 6 LRR (leucine-rich) repeats.

Gene Information

• Gene Name: RANGAP1 Ran GTPase activating protein 1 [ Homo sapiens ]
• Official Symbol: RANGAP1
• Synonyms: RANGAP1; Ran GTPase activating protein 1; SD, segregation distorter homolog (Drosophila); ran GTPase-activating protein 1; Fug1; KIAA1835
• Gene ID: 5905
• mRNA Refseq: NM_002883
• Protein Refseq: NP_002874
• MIM: 602362
• Uniprot ID: P46060
• Chromosome Location: 22q13
• Pathway: Cell Cycle, Mitotic, organism-specific biosystem; DNA Replication, organism-specific biosystem; HIV Infection, organism-specific biosystem; HIV Life Cycle, organism-specific biosystem; Host Interactions of HIV factors, organism-specific biosystem
• Function: GTPase activator activity; Ran GTPase activator activity; protein binding