Recombinant Human RASGRP2, His-tagged

• Cat.No.: RASGRP2-31042TH
• Product Overview: Recombinant fragment, corresponding to amino acids 230-609 of Human RASGRP2 with an N terminal His tag. Observed mwt: 50 kDa

Specification

• Description: The protein encoded by this gene is a brain-enriched nucleotide exchanged factor that contains an N-terminal GEF domain, 2 tandem repeats of EF-hand calcium-binding motifs, and a C-terminal diacylglycerol/phorbol ester-binding domain. This protein can activate small GTPases, including RAS and RAP1/RAS3. The nucleotide exchange activity of this protein can be stimulated by calcium and diacylglycerol. Three alternatively spliced transcript variants encoding the same protein have been found for this gene.
• Conjugation: HIS
• Source: E. coli
• Form: Lyophilised:Reconstitute with 124 μl aqua dest.
• Storage buffer: Preservative: NoneConstituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequences of amino acids: VAEKLLQLQNFNTLMAVVGGLSHSS ISRLKETHSHVSPET IKLWEGLTELVTATGNYGNYRRRLA ACVGFRFPILGVH LKDLVALQLALPDWLDPARTRLNGA KMKQLFSILEELAMVTSLRPPVQAN PDLLSLLTVSLDQYQTEDELYQLSL QRE PRSKSSPTSPTSCTPPPRPPVLEEW TSAAKPKLDQALV VEHIEKMVESVFRNFDVDGDGHISQ EEFQIIRGNFPYLSA FGDLDQNQDGCISREEMVSYFLRSS SVLGGRMGFVHNF QESNSLRPVACRHCKALILGIYKQG LKCRACGVNCHKQ CKDRLSVECRRRAQSVSLEGSAPSP SPMHSHHHRAFSFSL PRPGRRGSRPPEIREEEVQTVEDGV FDIHL

Gene Information

• Gene Name: RASGRP2 RAS guanyl releasing protein 2 (calcium and DAG-regulated) [ Homo sapiens ]
• Official Symbol: RASGRP2
• Synonyms: RASGRP2; RAS guanyl releasing protein 2 (calcium and DAG-regulated); RAS guanyl-releasing protein 2; CALDAG GEFI
• Gene ID: 10235
• mRNA Refseq: NM_001098670
• Protein Refseq: NP_001092140
• MIM: 605577
• Uniprot ID: Q7LDG7
• Chromosome Location: 11q13
• Pathway: Adaptive Immune System, organism-specific biosystem; Chemokine signaling pathway, organism-specific biosystem; Chemokine signaling pathway, conserved biosystem; Effects of PIP2 hydrolysis, organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem
• Function: calcium ion binding; calcium ion binding; diacylglycerol binding; guanyl-nucleotide exchange factor activity; lipid binding