Recombinant Human NEDD4, FLAG-tagged

• Cat.No.: NEDD4-520H
• Product Overview: Recombinant Human NEDD4 (amino-acids 150-end) was expressed in aBaculovirus infected Sf9cell expression system with N-terminal FLAG tag, MW= 86 kDa.

Specification

• Cat. No.: NEDD4-520H
• Description: E3 ubiquitin-protein ligase NEDD4 is an enzyme that in humans is encoded by the NEDD4 gene.
• Source: Sf9.
• Formulated In: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide, 20% glycerol, and 3 mM DTT.
• Application: Useful in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Purity: > 60%.
• Stability: >6 months at -80°C.

Gene Information

• Gene Name: NEDD4 neural precursor cell expressed, developmentally down-regulated 4 [ Homo sapiens ]
• Synonyms: neural precursor cell expressed, developmentally down-regulated 4; RPF1; NEDD4-1; KIAA0093; MGC176705; NEDD4; receptor-potentiating factor 1; EC 6.3.2.-; NEDD-4; Neural precursor cell expressed developmentally down-regulated protein 4
• Gene ID: 4734
• mRNA Refseq: NM_006154
• Protein Refseq: NP_006145
• MIM: 602278
• UniProt ID: P46934
• Chromosome Location: 15q
• Pathway: Endocytosis; Ubiquitin mediated proteolysis
• Function: RNA polymerase binding; acid-amino acid ligase activity; beta-2 adrenergic receptor binding; ligase activity; phosphoserine binding; ubiquitin binding ; phosphothreonine binding; proline-rich region binding; protein domain specific binding; sodium channel inhibitor activity; ubiquitin-protein ligase activity