Recombinant Human SPHK2, His-tagged

• Cat.No.: SPHK2-30668TH
• Product Overview: Full length recombinant human SPHK2 with N-terminal His tag.

Specification

• Description: This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
• Conjugation: HIS
• Biological activity: Specific Activity : 20 U/ug. One unit defined as the amount of enzyme that will transfer 1nmol phosphate to Tyr substrate per minute at pH 7.4 and 30deg.C. Assay buffer : 50mM HEPES pH 7.4, 3mM MgCl2, 3mM MnCl2, 1mM DTT, 3um Na-orthovanadate, 0.1M ATP, 30
• Form: Liquid
• Storage buffer: Preservative: NoneConstituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0
• Storage: Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
• Sequence Similarities: Contains 1 DAGKc domain.

Gene Information

• Gene Name: SPHK2 sphingosine kinase 2 [ Homo sapiens ]
• Official Symbol: SPHK2
• Synonyms: SPHK2; sphingosine kinase 2
• Gene ID: 56848
• mRNA Refseq: NM_001204158
• Protein Refseq: NP_001191087
• MIM: 607092
• Uniprot ID: Q9NRA0
• Chromosome Location: 19q13.2
• Pathway: Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Ceramide signaling pathway, organism-specific biosystem; Fc gamma R-mediated phagocytosis, organism-specific biosystem; Fc gamma R-mediated phagocytosis, conserved biosystem
• Function: ATP binding; D-erythro-sphingosine kinase activity; Ras GTPase binding; diacylglycerol kinase activity; nucleotide binding