Active Recombinant Human Phosphodiesterase 9A, GST-tagged

• Cat.No.: PDE9A-448H
• Product Overview: Recombinant Human PDE9A full length(Pro 181-Lys 50) fussed with GST tag at N-terminal was expressed in E. coli.

Specification

• Description: High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A, also known as PDE9A, is a member of the cyclic nucleotide phosphodiesterase family and PDE9 subfamily. PDE9A is expressed in all tissues examined (testis, brain, small intestine, skeletal muscle, heart, lung, thymus, spleen, placenta, kidney, liver, pancreas, ovary and prostate) except blood. Highest levels of PDE9A is in brain, heart, kidney, spleen, prostate and colon. Isoform PDE9A12 is found in prostate. PDE9A mRNA is widely distributed throughout the rat and mouse brain, with the highest expression observed in cerebellar Purkinje cells. PDE9A is the only cGMP-specific PDE with significant expression in the forebrain, and as such is likely to play an important role in NO-cGMP signaling. PDE9A is highly conserved between species and is widely distributed throughout the rodent brain. PDE9A is probably involved in maintenance of low cGMP levels in cells and might play an important role in a variety of brain functions involving cGMP-mediated signal transduction. PDE9A hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes. PDE9A represents a novel drug target worthy of further study.
• Source: Insect cells
• Species: Human
• Tag: GST
• Form: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 3 mM DTT and 20% glycerol
• Bio-activity: ≥1064 pmol/min/µg.
• Molecular Mass: MW=87.7 kDa
• Purity: ≥60%
• Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cGMP to 5'-GMP per minute at 37°C.
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Stability: >6 months at -80°C. Avoid freeze/thaw cycles.

Gene Information

• Gene Name: PDE9A phosphodiesterase 9A [ Homo sapiens ]
• Official Symbol: PDE9A
• Synonyms: HSPDE9A2; high affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A; CGMP-specific 3',5'-cyclic phosphodiesterase type 9; phosphodiesterase PDE9A21
• Gene ID: 5152
• mRNA Refseq: NM_001001567
• Protein Refseq: NP_001001567
• MIM: 602973
• UniProt ID: O76083
• Chromosome Location: 21q22.3
• Pathway: Hemostasis, organism-specific biosystem; Nitric oxide stimulates guanylate cyclase, organism-specific biosystem; Platelet homeostasis, organism-specific biosystem
• Function: 3',5'-cyclic-GMP phosphodiesterase activity; 3',5'-cyclic-nucleotide phosphodiesterase activity; metal ion binding