Recombinant Human PTPRG, GST-tagged

• Cat.No.: PTPRG-459H
• Product Overview: Recombinant Human PTPRG encoding the Catalytic domain (a.a. 801-1147) was expressed in anE.coliexpression system with N-terminal GST tag. MW=65.6 kDa.

Specification

• Cat. No.: PTPRG-459H
• Description: Receptor-type tyrosine-protein phosphatase gamma is an enzyme that in humans is encoded by the PTPRG gene. The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. The extracellular region of this PTP contains a carbonic anhydrase-like (CAH) domain, which is also found in the extracellular region of PTPRBETA/ZETA. This gene is located in a chromosomal region that is frequently deleted in renal cell carcinoma and lung carcinoma, thus is thought to be a candidate tumor suppressor gene.
• Source: E.Coli.
• Formulated In: 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione.
• Application: Useful for the study of enzyme kinetics, regulation, and to dephosphorylate target substrates.
• Specific Activity: 10 U/ug. One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 50 mM pNPP.
• Purity: > 95% by SDS-PAGE.
• Stability: >6 months at -80°C.

Gene Information

• Gene Name: PTPRG protein tyrosine phosphatase, receptor type, G [ Homo sapiens ]
• Synonyms: protein tyrosine phosphatase, receptor type, G; PTPG; HPTPG; RPTPG; R-PTP-GAMMA; PTPRG; H_RG317H01.1; protein tyrosine phosphatase gamma; receptor tyrosine phosphatase gamma; receptor-type protein phosphatase gamma; receptor type protein tyrosine phosphatase gamma; protein tyrosine phosphatase, receptor type, gamma polypeptide; EC 3.1.3.48; H_RG317H01.1; R-PTP-gamma; Protein-tyrosine phosphatase gamma
• Gene ID: 5793
• mRNA Refseq: NM_002841
• Protein Refseq: NP_002832
• MIM: 176886
• UniProt ID: P23470
• Chromosome Location: 3p21-p14
• Function: hydrolase activity; identical protein binding; transmembrane receptor protein tyrosine phosphatase activity