Recombinant Human TLR3 was expressed in HEK293.
Sterile filtered through a 0.2 micron filter. Lyophilized from 20 mM Sodium Phosphate, pH 7.5.
Determined by its ability to inhibit IL-8 secretion induced by Poly I:C in the TLR3 receptor-expressing HEK293 cells (HEK293 TLR3r). The ED50for this effect is ≤ 30 μg/ml in the presence of 100 μg/ml of Poly I:C.
STTKCTVSH EVADCSHLK LTQVPDDLP TNITVLNLT HNQLRRLPA ANFTRYSQL TSLDVGFNT ISKLEPELC QKLPMLKVL NLQHNELSQ LSDKTFAFC TNLTELHLM SNSIQKIKN NPFVKQKNL ITLDLSHNG LSSTKLGTQ VQLENLQEL LLSNNKIQA LKSEELDIF ANSSLKKLE LSSNQIKEF SPGCFHAIG RLFGLFLNN VQLGPSLTE KLCLELANT SIRNLSLSN SQLSTTSNT TFLGLKWTN LTMLDLSYN NLNVVGNDS FAWLPQLEY FFLEYNNIQ HLFSHSLHG LFNVRYLNL KRSFTKQSI SLASLPKID DFSFQWLKC LEHLNMEDN DIPGIKSNM FTGLINLKY LSLSNSFTS LRTLTNETF VSLAHSPLH ILNLTKNKI SKIESDAFS WLGHLEVLD LGLNEIGQE LTGQEWRGL ENIFEIYLS YNKYLQLTR NSFALVPSL QRLMLRRVA LKNVDSSPS PFQPLRNLT ILDLSNNNI ANINDDMLE GLEKLEILD LQHNNLARL WKHANPGGP IYFLKGLSH LHILNLESN GFDEIPVEV FKDLFELKI IDLGLNNLN TLPASVFNN QVSLKSLNL QKNLITSVE KKVFGPAFR NLTELDMRF NPFDCTCES IAWFVNWIN ETHTNIPEL SSHYLCNTP PHYHGFPVR LFDTSSCKD SAPFEL
Endotoxin level is <0.1 ng/ug of protein(1 EU/ug)
Above 95% as determined by SDS-PAGE Analysis.
Store at -20 centigrade
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein(example 0.1% BSA) and store in working aliquots at -20 centigrade to - 80 centigrade.