Recombinant Human TNFSF10, FLAG-tagged

• Cat.No.: TNFSF10-31604TH
• Product Overview: Recombinant fragment from the extracellular region of Human TRAIL (amino acids 95-281) with a N terminal DDDDK tag; MWt: 23kDa by SDS PAGE.

Specification

• Description: The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. This protein binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Protein length: 187 amino acids
• Conjugation: FLAG
• Molecular Weight: 23.000kDa inclusive of tags
• Source: E. coli
• Tissue specificity: Widespread; most predominant in spleen, lung and prostate.
• Biological activity: Induces apoptosis of Human Jurkat T cells in a concentration range of 5-50ng/ml in the presence of 1μg/ml TNF Ligands Enhancer. In the absence ofTNF Ligands Enhancer, TNFSF10-31604TH is poorly active.
• Form: Lyophilised:Reconstitute with 100μl sterile water. PBS containing at least 0.1% BSA should be used for further dilutions.
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: PBS
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: TSEETISTVQEKQQNISPLVRERGP QRVAAHITGTRGRSNTLSSPN SKNEKALGRKINSWESSRSGHSFLS NLHLRNGELVIHEKGFYYIYSQTYF RFQEEIKENTKNDKQMVQYIYKYTS YPDPILLMKSARNSCWSKDAEYGLY SIYQGGIFELKENDRIFVSVTNEHL IDMDHEASFFGAFLVG
• Sequence Similarities: Belongs to the tumor necrosis factor family.

Gene Information

• Gene Name: TNFSF10 tumor necrosis factor (ligand) superfamily, member 10 [ Homo sapiens ]
• Official Symbol: TNFSF10
• Synonyms: TNFSF10; tumor necrosis factor (ligand) superfamily, member 10; tumor necrosis factor ligand superfamily member 10; Apo 2L; CD253; TL2; TRAIL
• Gene ID: 8743
• mRNA Refseq: NM_001190942
• Protein Refseq: NP_001177871
• MIM: 603598
• Uniprot ID: P50591
• Chromosome Location: 3q26
• Pathway: Activation of Pro-Caspase 8, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis, conserved biosystem; Apoptosis, organism-specific biosystem
• Function: cytokine activity; metal ion binding; protein binding; receptor binding; tumor necrosis factor receptor binding