Recombinant Human SIRT1, GST-tagged
Cat.No. : | SIRT1-462H |
Product Overview : | Recombinant Human SIRT1 (amino acids 193- 741) was expressed in anE.coliexpression system with N-terminal GST tag. MW=87.2 kDa. |
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Cat. No. : | SIRT1-462H |
Description : | Sirtuin 1 also known as NAD-dependent deacetylase sirtuin-1 is a protein that in humans is encoded by the SIRT1 gene. SIRT1 stands for sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae), referring to the fact that its sirtuin homolog (biological equivalent across species) in yeast (S. cerevisiae) is Sir2. SIRT1 is an enzyme which deacetylates proteins that contribute to cellular regulation (reaction to stressors, longevity). |
Source : | E.Coli. |
Formulated In : | 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 20% glycerol. |
Application : | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
Specific Activity : | 8 pmol/min /μg. Assay condition: 25 mM Tris/Cl, pH8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 30 μM Biomol substrate (Catalog number KI-177), 200 μM NAD+and 20 ng/μl Sirtuin 1. Incubation condition: 30 min at 30ºC. |
Purity : | > 80%. |
Stability : | >6 months at –80°C. |
Publication : |
Circular and linear: a tale of aptamer selection for the activation of SIRT1 to induce death in cancer cells (2020)
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Gene Name : | SIRT1 sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) [ Homo sapiens ] |
Synonyms : | sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae); SIR2L1; SIRT1; sirtuin 1; SIR2alpha; sir2-like 1; sirtuin type 1; EC 3.5.1.-; OTTHUMP00000019691; OTTHUMP00000060745; SIR2alpha; hSIR2; hSIRT1; SIR2-like protein 1; sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) |
Gene ID : | 23411 |
mRNA Refseq : | NM_001142498 |
Protein Refseq : | NP_001135970 |
MIM : | 604479 |
UniProt ID : | Q96EB6 |
Chromosome Location : | 10q21.3 |
Function : | NAD binding; NAD+ ADP-ribosyltransferase activity; NAD-dependent histone deacetylase activity; deacetylase activity; histone binding; hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; identical protein binding; metal ion binding; p53 binding; protein C-terminus binding; protein domain specific binding; transcription corepressor activity; zinc ion binding |
Products Types
◆ Recombinant Protein | ||
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SIRT1-1165H | Recombinant Human SIRT1 protein(Met193-Ser747), His-tagged | +Inquiry |
SIRT1-30H | Recombinant Human SIRT1 Protein, GST-tagged | +Inquiry |
Sirt1-5883M | Recombinant Mouse Sirt1 Protein, Myc/DDK-tagged | +Inquiry |
SIRT1-2014H | Recombinant Human SIRT1 Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
SIRT1-595HCL | Recombinant Human SIRT1 lysate | +Inquiry |
◆ Assay kits | ||
Kit-2024 | SIRT1 Direct Fluorescent Screening Assay Kit | +Inquiry |
Kit-2025 | SIRT1 FRET-Based Screening Assay Kit | +Inquiry |
Kit-2156 | SIRT1 Inhibitor/Activator Screening Kit | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (6)
Ask a questionStudies have found that SIRT1 plays an important role in tissue regeneration. Activation of SIRT1 can promote the proliferation and differentiation of stem cells, which contributes to tissue regeneration and repair.
Activation of SIRT1 promotes autophagy, which helps to remove damaged organelles and proteins and maintain cell health.
SIRT1 regulates mitochondrial function. Activation of SIRT1 can improve mitochondrial metabolism and function, contributing to energy metabolism and cell survival.
Studies have found that SIRT1 exerts carcinogenic effects in some cancers, but exerts anti-cancer effects in others. It depends on the type of cancer and environmental factors.
SIRT1 has potential therapeutic value in drug development. The development of small molecule drugs targeting SIRT1 can be used to treat a variety of diseases, including aging, diabetes, cardiovascular diseases, and neurodegenerative diseases.
SIRT1 interacts and regulates with other sirtuins. For example, SIRT1 can regulate the expression and activity of other Sirtuins, and other Sirtuins can also regulate the activity of SIRT1.
Customer Reviews (3)
Write a reviewResulting banding is clear and bright, much more than expected.
SIRT1 has high safety, environmental protection and no pollution.
The quality is so good. It saves a lot of time. Good.
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