Recombinant Mouse Tgfbr1((Ala21-Glu121) & (Thr22-Glu121)) fused with an C-terminal Fc tag was expressed in Insect cells.
Predicted N Terminal:
Ala21 & Thr22
Lyophilized from a 0.2 μm filtered solution in PBS.
Measured by its binding ability in a functional ELISA. When Recombinant Rat Endoglin/CD105 Fc Chimera or Recombinant Mouse Endoglin/CD105 Fc Chimera is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse TGF-beta RI/ALK-5 Fc Chimera that produces 50% of the optimal binding response is found to be approximately 0.25-1.25 μg/mL.
Predicted Molecular Mass: 37.5 (monomer) kDa;
SDS-PAGE: 40-50 kDa, reducing conditions.
<0.10 EU per 1 μg of the protein by the LAL method.
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitute at 500 μg/mL in sterile PBS.