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Active Recombinant Mouse Vcam1, His & hFc-tagged

Cat.No. : Vcam1-1784M
Product Overview : Recombinant Mouse Vcam1(Met 1-Glu 698)(NP_035823.3) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
  • Specification
  • Gene Information
  • Related Products
Source : HEK293
Species : Mouse
Tag : His & hFc
Form : Lyophilized from sterile 50 mM Tris, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity : Measured by the ability of the immobilized protein to support adhesion of U937 human histiocytic lymphoma cells.
When cells are added to VCAM1-coated plates (10 μg/ml, 100 μg/well), approximately >70% cells will adhere specifically.
Molecular Mass : The recombinant mouse VCAM1/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 922 amino acids and has a predicted molecular mass of 102 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmVCAM1/Fc monomer is approximately 110-120 kDa due to glycosylation.
Protein Length : Met 1-Glu 698
Endotoxin : < 1.0 EU per μg protein as determined by the LAL method.
Purity : > 90 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
Gene Name : Vcam1 vascular cell adhesion molecule 1 [ Mus musculus ]
Official Symbol : Vcam1
Synonyms : VCAM1; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; V-CAM 1; CD106; Vcam-1;
Gene ID : 22329
mRNA Refseq : NM_011693
Protein Refseq : NP_035823

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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