Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Recombinant Mouse Wingless-related MMTV Integration Site 3A

Cat.No. : Wnt3a-1788M
Product Overview : A DNA sequence encoding the signal peptide from human CD33, Met 1 - Ala 16, joined with the mature mouse Wnt-3A, Ser 19 - Lys 352 was inserted into a suitable mammalian expression vector and expressed inCHOcells.
  • Specification
  • Gene Information
  • Related Products
Cat. No. : Wnt3a-1788M
Description : Wingless-type MMTV integration site family, member 3A, also known as WNT3A is an intercellular signaling molecule. WNT3A is expressed extensively in cells fated to give rise to embryonic mesoderm. Wnt-3a regulates survival, expansion, and maintenance of neural progenitors derived from embryonic stem cells. It has been reported that Wnt-3a supports self-renewal of embryonic stem cells and can maintain these cells in a pluripotent state. Wnt-3a is also considered a ligand that activates the beta-catenin-dependent pathway in Wnt signaling, which is implicated in numerous physiological events such as morphogenesis.
Molecular Weight : The predicted molecular weight of Recombinant Mouse WNT-3a is Mr 37 kDa. However, because of glycosylation the actual molecular weight as observed by migration on SDS Page is Mr 41 kDa.
Source : CHO.
State Of Matter : Lyophilized.
Purity : >75% as determined by SDS-PAGE and visualized by silver stain.
Endotoxin : <1.0 EU/µg as determined by the LAL method.
Biological Activity : The biological activity of Mouse WNT-3a was determined by its ability to synergize with BMP-2 to induce alkaline phosphatase synthesis by MC3T3E1 osteoblastic cells. The expected ED50 is typically 2 - 10 ng/mL, in the presence of a low or non-inducing amount of recombinant human BMP-2 (5 - 15 ng/mL). Approximately 50 - 100 ng/mL results in maximal synergy with BMP-2 activity.
Storage And Stability : This lyophilized protein is stable for six to twelve months when stored desiccated at -20°Cto -70°C. After aseptic reconstitution, this protein may be stored at 2°Cto 8°Cfor one month or at -20°Cto -70°Cin a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Gene Name : Wnt3a wingless-related MMTV integration site 3A [ Mus musculus ]
Synonyms : Wnt3a; wingless-related MMTV integration site 3A; vt; Wnt-3a; vestigial tail; OTTMUSP00000005961
Gene ID : 22416
mRNA Refseq : NM_009522
Protein Refseq : NP_033548
UniProt ID : P27467
Chromosome Location : 11 32.0 cM
Pathway : Basal cell carcinoma; Hedgehog signaling pathway; Melanogenesis; Pathways in cancer; Wnt signaling pathway
Function : receptor binding; protein binding; signal transducer activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (6)

Ask a question
Does CDH16 protein interact with WNT3A protein? 12/14/2019

No interaction between CDH16 protein and WNT3A protein has been found.

Which role of WNT3A protein in embryonic development? 09/15/2019

The WNT3A protein plays an important role in embryonic development. It is involved in the formation of multiple embryonic organs, directed cell migration, and the establishment of tissue polarity.

Have regulators of WNT3A protein been identified? 08/24/2019

Yes, multiple factors have been found in the WNT signaling pathway that regulate the expression and function of WNT3A protein, including Axin, APC, etc.

Where is the WNT3A protein in the Wnt signaling pathway? 05/18/2019

The WNT3A protein is in the starting position in the Wnt signaling pathway, and it is able to bind to the Wnt receptor and initiate the signaling cascade.

What is the relationship between WNT3A protein and cancer stem cells? 05/04/2019

WNT3A protein is closely related to cancer stem cells. It may be involved in the self-renewal and differentiation regulation of cancer stem cells, which is of great significance for tumor development and treatment.

How about the current clinical research progress on WNT3A protein? 03/28/2019

Clinical research on WNT3A protein is still ongoing. Studies have shown the important role of WNT3A protein in tumors, neurological diseases, bone metabolism diseases, etc., and future research will further explore its application potential in disease diagnosis, treatment and prognosis assessment.

Customer Reviews (3)

Write a review
Reviews
07/15/2022

    This protein is highly expressed and can efficiently synthesize a large number of target proteins in host cells.

    06/03/2022

      This protein has therapeutic effects on specific disease models and is a promising candidate for new drugs.

      11/20/2019

        The laboratory personnel feedback that the protein is easy to operate, can meet the experimental needs, and has a good cost performance.

        Ask a Question for All Wnt3a Products

        Required fields are marked with *

        My Review for All Wnt3a Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends