Recombinant Mouse Wingless-related MMTV Integration Site 3A
Cat.No. : | Wnt3a-1788M |
Product Overview : | A DNA sequence encoding the signal peptide from human CD33, Met 1 - Ala 16, joined with the mature mouse Wnt-3A, Ser 19 - Lys 352 was inserted into a suitable mammalian expression vector and expressed inCHOcells. |
- Specification
- Gene Information
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Cat. No. : | Wnt3a-1788M |
Description : | Wingless-type MMTV integration site family, member 3A, also known as WNT3A is an intercellular signaling molecule. WNT3A is expressed extensively in cells fated to give rise to embryonic mesoderm. Wnt-3a regulates survival, expansion, and maintenance of neural progenitors derived from embryonic stem cells. It has been reported that Wnt-3a supports self-renewal of embryonic stem cells and can maintain these cells in a pluripotent state. Wnt-3a is also considered a ligand that activates the beta-catenin-dependent pathway in Wnt signaling, which is implicated in numerous physiological events such as morphogenesis. |
Molecular Weight : | The predicted molecular weight of Recombinant Mouse WNT-3a is Mr 37 kDa. However, because of glycosylation the actual molecular weight as observed by migration on SDS Page is Mr 41 kDa. |
Source : | CHO. |
State Of Matter : | Lyophilized. |
Purity : | >75% as determined by SDS-PAGE and visualized by silver stain. |
Endotoxin : | <1.0 EU/µg as determined by the LAL method. |
Biological Activity : | The biological activity of Mouse WNT-3a was determined by its ability to synergize with BMP-2 to induce alkaline phosphatase synthesis by MC3T3E1 osteoblastic cells. The expected ED50 is typically 2 - 10 ng/mL, in the presence of a low or non-inducing amount of recombinant human BMP-2 (5 - 15 ng/mL). Approximately 50 - 100 ng/mL results in maximal synergy with BMP-2 activity. |
Storage And Stability : | This lyophilized protein is stable for six to twelve months when stored desiccated at -20°Cto -70°C. After aseptic reconstitution, this protein may be stored at 2°Cto 8°Cfor one month or at -20°Cto -70°Cin a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions. |
Gene Name : | Wnt3a wingless-related MMTV integration site 3A [ Mus musculus ] |
Synonyms : | Wnt3a; wingless-related MMTV integration site 3A; vt; Wnt-3a; vestigial tail; OTTMUSP00000005961 |
Gene ID : | 22416 |
mRNA Refseq : | NM_009522 |
Protein Refseq : | NP_033548 |
UniProt ID : | P27467 |
Chromosome Location : | 11 32.0 cM |
Pathway : | Basal cell carcinoma; Hedgehog signaling pathway; Melanogenesis; Pathways in cancer; Wnt signaling pathway |
Function : | receptor binding; protein binding; signal transducer activity |
Products Types
◆ Recombinant Protein | ||
WNT3A-116H | Recombinant Human WNT3A Protein | +Inquiry |
WNT3A-55H | Active Recombinant Human WNT3A Protein, Animal Free | +Inquiry |
Wnt3a-56M | Active Recombinant Mouse Wnt3a Protein | +Inquiry |
WNT3A-10196M | Recombinant Mouse WNT3A Protein, His (Fc)-Avi-tagged | +Inquiry |
Wnt3a-585M | Recombinant Mouse Wnt3a Protein, His-tagged | +Inquiry |
◆ Lysates | ||
WNT3A-295HCL | Recombinant Human WNT3A 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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- Q&As
- Reviews
Q&As (6)
Ask a questionNo interaction between CDH16 protein and WNT3A protein has been found.
The WNT3A protein plays an important role in embryonic development. It is involved in the formation of multiple embryonic organs, directed cell migration, and the establishment of tissue polarity.
Yes, multiple factors have been found in the WNT signaling pathway that regulate the expression and function of WNT3A protein, including Axin, APC, etc.
The WNT3A protein is in the starting position in the Wnt signaling pathway, and it is able to bind to the Wnt receptor and initiate the signaling cascade.
WNT3A protein is closely related to cancer stem cells. It may be involved in the self-renewal and differentiation regulation of cancer stem cells, which is of great significance for tumor development and treatment.
Clinical research on WNT3A protein is still ongoing. Studies have shown the important role of WNT3A protein in tumors, neurological diseases, bone metabolism diseases, etc., and future research will further explore its application potential in disease diagnosis, treatment and prognosis assessment.
Customer Reviews (3)
Write a reviewThis protein is highly expressed and can efficiently synthesize a large number of target proteins in host cells.
This protein has therapeutic effects on specific disease models and is a promising candidate for new drugs.
The laboratory personnel feedback that the protein is easy to operate, can meet the experimental needs, and has a good cost performance.
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