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Recombinant GST-alpha Spectrin

Cat.No. : GST-52
Product Overview : Recombinant fusion protein: Glutathione-S-Transferase (GST) and a fragment of human α-spectrin (from Lysine 1601 to Leucine 1818) containing repeat 17 and the ubiquitination target lysine 27. The total molecular weight of the fusion protein is 52.4 kDa.
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Cat. No. : GST-52
Description : Erythrocyte spectrin is the main component of the red cell membrane skeleton responsible for the shape and physical properties of red cells. Spectrin is composed of 2 subunits and (280 and 246 kDa respectively). The human erithroid α-spectrin (2418 amino acids) consists of 22 repeating segments of about 106 amino acids in length. This protein was demonstrated to be a specific substrate for ubiquitina tion in vitro and in vivo. The main ubiquitination site on human α-spectrin was identified in the lysine 27 of the repeating segment 17 by sitedirected mutagenesis. Cell-free experiments using radiolabeled, biotinylated or native ubiquitin and cellular lysates from rabbit retyculocytes, human erythrocytes or k562 cells, showed that only monoubiquitination occurs at this site.
Source : Escherichia Coli.
Physical Appearance : Sterile Filtered clear solution.
Purity : Greater than 90.0% as determined by both: (a) Analysis by RP-HPLC. (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel
Formulation : The protein (1.7mg/ml) contains 50mM Tris buffer pH-8 and 10mM glutathione.
Application : Substrate for cell-free ubiquitination assays.
Storage : rGST-alpha Spectrin although stable at 14℃ for 1 week, should be stored desiccated below -18℃. Please prevent freeze-thaw cycles.
Synonyms : GST-a Spectrin; GST-alpha Spectrin.
Synonyms : GST-a Spectrin; GST-alpha Spectrin.

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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