CRABP1 (cellular retinoic acid-binding protein 1) is a member of specific carrier proteins for members of the vitamin A family. CRABP1 is assumed to play an important role in retinoic acid-mediated differentiation and proliferation processes. CRABP1 is structurally similar to the cellular retinol-binding proteins, but binds only retinoic acid. It is constitutively expressed and is believed to have different functions in the cell than the related CRABP2. Interestingly, Sytenol A bakuchiol has very specific receptor specificity over retinol and has no effect on the RAR-beta and RAR-gamma receptors and down-regulates CRABP1.
Recombinant human CRABP1 (1-137aa) purified from E. coli.
Mouse IgG2b heavy chain and k light chain.
Anti-human CRABP1 mAb, clone AT1A1, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CRABP1 protein.
Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.
Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1000. Recommended starting dilution is 1:500.
Western Blot Analysis:
The extracts of mouse eye (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CRABP1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.