Active Recombinant Human ACACB protein, His-tagged

• Cat.No.: ACACB-1849H
• Product Overview: Recombinant full length Human ACACB fused with His tag at N-terminal was expressed in Insect cells.

Specification

• Description: Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. ACC-beta is thought to control fatty acid oxidation by means of the ability of malonyl-CoA to inhibit carnitine-palmitoyl-CoA transferase I, the rate-limiting step in fatty acid uptake and oxidation by mitochondria. ACC-beta may be involved in the regulation of fatty acid oxidation, rather than fatty acid biosynthesis. There is evidence for the presence of two ACC-beta isoforms.
• Source: Insect cells
• Species: Human
• Tag: His
• Form: 40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and 3 mM DTT.
• Bio-activity: ≥41 pmoles/min/µg
• Molecular Mass: 277 kDa
• Purity: >56%
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Storage: >6 months at –80 centigrade. Avoid freeze/thaw cycles.
• Concentration: 0.07 mg/ml

Gene Information

• Gene Name: ACACB acetyl-CoA carboxylase beta [ Homo sapiens ]
• Official Symbol: ACACB
• Synonyms: ACACB; acetyl-CoA carboxylase beta; acetyl Coenzyme A carboxylase beta; acetyl-CoA carboxylase 2; ACC2; ACCB; acetyl CoA carboxylase 2; HACC275; ACC-beta; acetyl-Coenzyme A carboxylase beta
• Gene ID: 32
• mRNA Refseq: NM_001093
• Protein Refseq: NP_001084
• MIM: 601557
• UniProt ID: O00763
• Chromosome Location: 12q24.1
• Pathway: AMPK signaling, organism-specific biosystem; Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; ChREBP activates metabolic gene expression, organism-specific biosystem; Fatty Acid Biosynthesis, organism-specific biosystem; Fatty acid biosynthesis, organism-specific biosystem; Fatty acid biosynthesis, conserved biosystem
• Function: ATP binding; acetyl-CoA carboxylase activity; biotin carboxylase activity; ligase activity; metal ion binding; nucleotide binding; protein binding