Active Recombinant Human BRAF/p50 Protein, Flag-His-tagged

• Cat.No.: BRAF-550H
• Product Overview: Recombinant Human BRAF (GenBank accession # NM_004333), a.a. 432-725 with N-terminal His-tag, MW=36 kDa, and p50 (GenBank accession # NM_007065), a.a. 2-378(end) with N-terminal FLAG-tag, MW=45 kDa, coexpressed in a Baculovirus infected Sf9 cell expression system. This protein exists in a complex with Cdc37 and p50.

Specification

• Description: This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008]
• Source: Insects cell
• Species: Human
• Tag: N-terminal His-tag (BRAF), N-terminal FLAG-tag (p50)
• Bio-activity: <1 pmol/min/μg.
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Stability: >6 months at –80 centigrade. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).
• Concentration: 0.09 mg/ml
• Storage Buffer: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 mM imidazole, 10% glycerol, and 3 mM DTT.
• Warning: Avoid freeze/thaw cycles

Gene Information

• Gene Name: BRAF B-Raf proto-oncogene, serine/threonine kinase [ Homo sapiens (human) ]
• Official Symbol: BRAF
• Synonyms: BRAF; B-Raf proto-oncogene, serine/threonine kinase; B-RAF1; RAFB1; cdc37; p50; NS7; B-raf; BRAF1; RAFB1; B-RAF1; serine/threonine-protein kinase B-raf; 94 kDa B-raf protein; B-Raf proto-oncogene serine/threonine-protein kinase (p94); B-Raf serine/threonine-protein; murine sarcoma viral (v-raf) oncogene homolog B1; proto-oncogene B-Raf; v-raf murine sarcoma viral oncogene homolog B; v-raf murine sarcoma viral oncogene homolog B1; EC 2.7.11.1
• Gene ID: 673
• mRNA Refseq: NM_004333
• Protein Refseq: NP_004324
• MIM: 164757
• UniProt ID: P15056