Mouse Anti-Rat Cd2 Monoclonal Antibody

Silencing of EcR-B1 in clones of follicle cells causes caspase-3 activation and Diap1 downregulation. Confocal images of stage 10B mosaic egg chambers expressing the UAS-IR-EcR-B1 transgene (A– H′ ). Double immunolabeling with anti-active caspase-3 (B and B′ ; green) and anti-CD2 (C and C′ ; cyan) coupled with propidium iodide staining (A and A′ ; red) shows that in clones expressing the EcR-B1 interfering transgene, follicle cells with highly condensed nuclei and active caspase-3 are recognizable (D and D′ ; merge of all signals). Egg chamber from females of the same genotype immunostained with anti-Diap1 (F and F′ ; green), anti-cleaved caspase-3 (G and G′ ; cyan) and stained with propidium iodide (E and E′ ; red). H and H′ are merged images of all signals. The brackets in E– H point to a wide area of strongly decreased Diap1 staining in follicular epithelium (F). In this area, follicle cells showing highly condensed nuclei (E) and active caspase-3 (G) are detectable. Higher magnification views of the boxed areas in E– H are shown in E′ – H′ where a dotted line marks the border between very low levels (left) and high levels (right) of Diap1 staining. Anterior is left in all panels. Bars, 20 μ m.