Active Recombinant Human Cystatin C

• Cat.No.: CST3-1914H
• Product Overview: Recombinant Human Cystatin C was expressed inhuman 293 cellsas a monomer with an apparent molecular mass of 12 to 13 kDa.

Specification

• Cat. No.: CST3-1914H
• Description: Native Cystatin C in human urine is found in two different forms: one with pI 9.2 and the other with pI 7.8 by elimination of small basic peptides or amino acids from the N-terminal end of protein. Cystatin C has been studied for its role in predicting newonset or deteriorating cardiovascular disease. This cytokine is produced in a serum-free, chemically defined media.
• Source: Human 293 cells.
• Activity: 0.3 to 1.5 μM IC50.
• Purity: >95%.
• Endotoxin: Endotoxin-free.
• Molecular Mass: 12 to 13 kDa, monomer, nonglycosylated.
• Purity Confirmation: The protein was resolved by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.
• Activity Assay: The inhibitory function of Cystatin C on papin's protease activity was measured by acolorimetric assay using L-BAPA as substrate. IC50 value was measured at 5 to 20 μg/mL (0.3 to 1.5 μM) with a range of 1.56μg/mL to 50μg/mL Cystatin C in presence of 0.55μM papain and 0.44μM L-BAPA.

Gene Information

• Gene Name: CST3 cystatin C [ Homo sapiens ]
• Synonyms: CST3; cystatin C; ARMD11; MGC117328; CST3; cystatin C (amyloid angiopathy and cerebral hemorrhage); cystatin C1; cystatin 3; Neuroendocrine basic polypeptide2; OTTHUMP000001641822; ARMD112; post-gamma-globulin; bA218C14.4 (cystatin C); OTTHUMP00000164181; Gamma-trace; gamma-trace; Post-gamma-globulin; MGC117328; Cystatin-3
• Gene ID: 1471
• mRNA Refseq: NM_000099
• Protein Refseq: NP_000090
• MIM: 604312
• UniProt ID: P01034
• Chromosome Location: 20p11.21
• Function: beta-amyloid binding; cysteine-type endopeptidase inhibitor activity; peptidase inhibitor activity; protease binding; protein binding